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| | ==Structure of a bacterial energy-coupling factor transporter== | | ==Structure of a bacterial energy-coupling factor transporter== |
| - | <StructureSection load='4hzu' size='340' side='right' caption='[[4hzu]], [[Resolution|resolution]] 3.53Å' scene=''> | + | <StructureSection load='4hzu' size='340' side='right'caption='[[4hzu]], [[Resolution|resolution]] 3.53Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4hzu]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_casei_g"_von_freudenreich_and_thoni_1904 "bacillus casei g" von freudenreich and thoni 1904]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HZU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HZU FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4hzu]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Levilactobacillus_brevis Levilactobacillus brevis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HZU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4HZU FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ecfA1, cbiO1, LVIS_1661 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1580 "Bacillus casei g" von Freudenreich and Thoni 1904]), ecfA2, cbiO2, LVIS_1662 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1580 "Bacillus casei g" von Freudenreich and Thoni 1904]), ecfT, LVIS_1660 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1580 "Bacillus casei g" von Freudenreich and Thoni 1904]), LVIS_2151 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1580 "Bacillus casei g" von Freudenreich and Thoni 1904])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.53Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hzu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hzu OCA], [http://pdbe.org/4hzu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4hzu RCSB], [http://www.ebi.ac.uk/pdbsum/4hzu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4hzu ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4hzu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hzu OCA], [https://pdbe.org/4hzu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4hzu RCSB], [https://www.ebi.ac.uk/pdbsum/4hzu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4hzu ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/ECFA1_LACBA ECFA1_LACBA]] Part of a common energy-coupling factor (ECF) ABC-transporter complex. Unlike classic ABC transporters this ECF transporter provides the energy necessary to transport a number of different substrates (By similarity). [[http://www.uniprot.org/uniprot/ECFT_LACBA ECFT_LACBA]] Transmembrane (T) component of an energy-coupling factor (ECF) ABC-transporter complex. Unlike classic ABC transporters this ECF transporter provides the energy necessary to transport a number of different substrates (By similarity). [[http://www.uniprot.org/uniprot/ECFA2_LACBA ECFA2_LACBA]] Part of a common energy-coupling factor (ECF) ABC-transporter complex. Unlike classic ABC transporters this ECF transporter provides the energy necessary to transport a number of different substrates (By similarity). | + | [https://www.uniprot.org/uniprot/ECFA2_LEVBA ECFA2_LEVBA] ATP-binding (A) component of a common energy-coupling factor (ECF) ABC-transporter complex. Unlike classic ABC transporters this ECF transporter provides the energy necessary to transport a number of different substrates.[HAMAP-Rule:MF_01710] |
| - | <div style="background-color:#fffaf0;">
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| - | == Publication Abstract from PubMed ==
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| - | The energy-coupling factor (ECF) transporters constitute a novel family of conserved membrane transporters in prokaryotes that have a similar domain organization to the ATP-binding cassette transporters. Each ECF transporter comprises a pair of cytosolic ATPases (the A and A' components, or EcfA and EcfA'), a membrane-embedded substrate-binding protein (the S component, or EcfS) and a transmembrane energy-coupling component (the T component, or EcfT) that links the EcfA-EcfA' subcomplex to EcfS. The structure and transport mechanism of the quaternary ECF transporter remain largely unknown. Here we report the crystal structure of a nucleotide-free ECF transporter from Lactobacillus brevis at a resolution of 3.5 A. The T component has a horseshoe-shaped open architecture, with five alpha-helices as transmembrane segments and two cytoplasmic alpha-helices as coupling modules connecting to the A and A' components. Strikingly, the S component, thought to be specific for hydroxymethyl pyrimidine, lies horizontally along the lipid membrane and is bound exclusively by the five transmembrane segments and the two cytoplasmic helices of the T component. These structural features suggest a plausible working model for the transport cycle of the ECF transporters.
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| - | Structure of a bacterial energy-coupling factor transporter.,Wang T, Fu G, Pan X, Wu J, Gong X, Wang J, Shi Y Nature. 2013 May 9;497(7448):272-6. doi: 10.1038/nature12045. Epub 2013 Apr 14. PMID:23584587<ref>PMID:23584587</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
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| - | <div class="pdbe-citations 4hzu" style="background-color:#fffaf0;"></div>
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| - | == References ==
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| - | <references/>
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bacillus casei g von freudenreich and thoni 1904]] | + | [[Category: Large Structures]] |
| - | [[Category: Fu, G B]] | + | [[Category: Levilactobacillus brevis]] |
| - | [[Category: Pan, X J]] | + | [[Category: Fu GB]] |
| - | [[Category: Shi, Y G]] | + | [[Category: Pan XJ]] |
| - | [[Category: Wang, T L]] | + | [[Category: Shi YG]] |
| - | [[Category: Ecf]] | + | [[Category: Wang TL]] |
| - | [[Category: Hydrolase]]
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| - | [[Category: Membrane protein]]
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| - | [[Category: Transport protein]]
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| - | [[Category: Transporter]]
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