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4i4p

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==BEL beta-trefoil apo crystal form 2==
==BEL beta-trefoil apo crystal form 2==
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<StructureSection load='4i4p' size='340' side='right' caption='[[4i4p]], [[Resolution|resolution]] 1.28&Aring;' scene=''>
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<StructureSection load='4i4p' size='340' side='right'caption='[[4i4p]], [[Resolution|resolution]] 1.28&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4i4p]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Boletus_edulis Boletus edulis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4I4P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4I4P FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4i4p]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Boletus_edulis Boletus edulis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4I4P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4I4P FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.279&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4i4o|4i4o]], [[4i4q|4i4q]], [[4i4r|4i4r]], [[4i4s|4i4s]], [[4i4u|4i4u]], [[4i4v|4i4v]], [[4i4x|4i4x]], [[4i4y|4i4y]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4i4p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4i4p OCA], [http://pdbe.org/4i4p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4i4p RCSB], [http://www.ebi.ac.uk/pdbsum/4i4p PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4i4p ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4i4p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4i4p OCA], [https://pdbe.org/4i4p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4i4p RCSB], [https://www.ebi.ac.uk/pdbsum/4i4p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4i4p ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/R4GRU5_BOLED R4GRU5_BOLED]
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A novel lectin was purified from the fruiting bodies of king bolete mushrooms (Boletus edulis, also called porcino, cep or penny bun). The lectin was structurally characterized i.e its amino acid sequence and three-dimensional structure were determined. The new protein is a homodimer and each protomer folds as beta-trefoil domain and therefore we propose the name Boletus edulis lectin (BEL) beta-trefoil to distinguish it from the other lectin that has been described in these mushrooms. The lectin has potent anti-proliferative effects on human cancer cells, which confers to it an interesting therapeutic potential as an antineoplastic agent. Several crystal forms of the apoprotein and of complexes with different carbohydrates were studied by X-ray diffraction. The structure of the apoprotein was solved at 1.12 A resolution. The interaction of the lectin with lactose, galactose, N-acetylgalactosamine and T-antigen disaccharide, Galbeta1-3GalNAc, was examined in detail. All the three potential binding sites present in the beta-trefoil fold are occupied in at least one crystal form and are described in detail in this paper. No important conformational changes are observed in the lectin when comparing its co-crystals with carbohydrates with those of the ligand-free protein.
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BEL beta-trefoil: A novel lectin with antineoplastic properties in king bolete (Boletus edulis) mushrooms.,Bovi M, Cenci L, Perduca M, Capaldi S, Carrizo ME, Civiero L, Chiarelli LR, Galliano M, Monaco HL Glycobiology. 2013 May;23(5):578-92. doi: 10.1093/glycob/cws164. Epub 2012 Dec 4. PMID:23213111<ref>PMID:23213111</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4i4p" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Boletus edulis]]
[[Category: Boletus edulis]]
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[[Category: Bovi, M]]
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[[Category: Large Structures]]
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[[Category: Capaldi, S]]
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[[Category: Bovi M]]
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[[Category: Carrizo, M E]]
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[[Category: Capaldi S]]
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[[Category: Cenci, L]]
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[[Category: Carrizo ME]]
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[[Category: Chiarelli, L R]]
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[[Category: Cenci L]]
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[[Category: Civiero, L]]
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[[Category: Chiarelli LR]]
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[[Category: Galliano, M]]
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[[Category: Civiero L]]
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[[Category: Monaco, H L]]
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[[Category: Galliano M]]
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[[Category: Perduca, M]]
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[[Category: Monaco HL]]
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[[Category: Fruiting body]]
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[[Category: Perduca M]]
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[[Category: Lectin]]
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[[Category: Sugar binding protein]]
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Current revision

BEL beta-trefoil apo crystal form 2

PDB ID 4i4p

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