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4i88

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==R107G HSP16.5==
==R107G HSP16.5==
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<StructureSection load='4i88' size='340' side='right' caption='[[4i88]], [[Resolution|resolution]] 2.85&Aring;' scene=''>
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<StructureSection load='4i88' size='340' side='right'caption='[[4i88]], [[Resolution|resolution]] 2.85&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4i88]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Metja Metja]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4I88 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4I88 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4i88]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii_DSM_2661 Methanocaldococcus jannaschii DSM 2661]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4I88 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4I88 FirstGlance]. <br>
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</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MJ0285 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=243232 METJA])</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.85&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4i88 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4i88 OCA], [http://pdbe.org/4i88 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4i88 RCSB], [http://www.ebi.ac.uk/pdbsum/4i88 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4i88 ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4i88 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4i88 OCA], [https://pdbe.org/4i88 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4i88 RCSB], [https://www.ebi.ac.uk/pdbsum/4i88 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4i88 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/HSPS_METJA HSPS_METJA]] Chaperone that confers thermal protection to other proteins.
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[https://www.uniprot.org/uniprot/HSPS_METJA HSPS_METJA] Chaperone that confers thermal protection to other proteins.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The archael small heat-shock protein (sHSP), MjHSP16.5, forms a 24-subunit oligomer with octahedral symmetry. Here, we demonstrate that the IXI motif present in the C-terminal domain is necessary for the oligomerization of MjHSP16.5. Removal increased the in vitro chaperone activity with citrate synthase as the client protein. Less predictable were the effects of the R107G substitution in MjHSP16.5 because of the differences in the oligomerization of metazoan and non-metazoan sHSPs. We present the crystal structure for MjHSP16.5 R107G and compare this with an improved (2.5 A) crystal structure for wild-type (WT) MjHSP16.5. Although no significant structural differences were found in the crystal, using cryo-electron microscopy, we identified two 24mer species with octahedral symmetry for the WT MjHSP16.5 both at room temperature and at 60 degrees C, all showing two major species with the same diameter of 12.4 nm. Similarly, at room temperature, there are also two kinds of 12.4 nm oligomers for R107G MjHSP16.5, but in the 60 degrees C sample, a larger 24mer species with a diameter of 13.6 nm was observed with significant changes in the fourfold symmetry axis and dimer-dimer interface. This highly conserved arginine, therefore, contributes to the quaternary organization of non-metazoan sHSP oligomers. Potentially, the R107G substitution has functional consequences as R107G MjHSP16.5 was far superior to the WT protein in protecting betaL-crystallin against heat-induced aggregation.
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Changes in the quaternary structure and function of MjHSP16.5 attributable to deletion of the IXI motif and introduction of the substitution, R107G, in the alpha-crystallin domain.,Quinlan RA, Zhang Y, Lansbury A, Williamson I, Pohl E, Sun F Philos Trans R Soc Lond B Biol Sci. 2013 Mar 25;368(1617):20120327. doi:, 10.1098/rstb.2012.0327. Print 2013 May 5. PMID:23530263<ref>PMID:23530263</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4i88" style="background-color:#fffaf0;"></div>
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==See Also==
==See Also==
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*[[Heat Shock Proteins|Heat Shock Proteins]]
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*[[Heat Shock Protein structures|Heat Shock Protein structures]]
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Metja]]
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[[Category: Large Structures]]
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[[Category: Pohl, E]]
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[[Category: Methanocaldococcus jannaschii DSM 2661]]
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[[Category: Quinlan, R A]]
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[[Category: Pohl E]]
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[[Category: Williamson, I R]]
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[[Category: Quinlan RA]]
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[[Category: Alpha-b domain]]
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[[Category: Williamson IR]]
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[[Category: Chaperone]]
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Current revision

R107G HSP16.5

PDB ID 4i88

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