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4j7o

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==Structure of the N-terminal Repeat Domain of Rickettsia Sca2==
==Structure of the N-terminal Repeat Domain of Rickettsia Sca2==
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<StructureSection load='4j7o' size='340' side='right' caption='[[4j7o]], [[Resolution|resolution]] 2.17&Aring;' scene=''>
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<StructureSection load='4j7o' size='340' side='right'caption='[[4j7o]], [[Resolution|resolution]] 2.17&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4j7o]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Rickettsia_conorii Rickettsia conorii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J7O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4J7O FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4j7o]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rickettsia_conorii Rickettsia conorii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J7O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4J7O FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.175&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">sca2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=781 Rickettsia conorii])</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4j7o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4j7o OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4j7o RCSB], [http://www.ebi.ac.uk/pdbsum/4j7o PDBsum]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4j7o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4j7o OCA], [https://pdbe.org/4j7o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4j7o RCSB], [https://www.ebi.ac.uk/pdbsum/4j7o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4j7o ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/SCA2_RICCN SCA2_RICCN]
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Sca2 (surface cell antigen 2) is the only bacterial protein known to promote both actin filament nucleation and profilin-dependent elongation, mimicking eukaryotic formins to assemble actin comet tails for Rickettsia motility. We show that Sca2's functional mimicry of formins is achieved through a unique mechanism. Unlike formins, Sca2 is monomeric, but has N- and C-terminal repeat domains (NRD and CRD) that interact with each other for processive barbed-end elongation. The crystal structure of NRD reveals a previously undescribed fold, consisting of helix-loop-helix repeats arranged into an overall crescent shape. CRD is predicted to share this fold and might form together with NRD, a doughnut-shaped formin-like structure. In between NRD and CRD, proline-rich sequences mediate the incorporation of profilin-actin for elongation, and WASP-homology 2 (WH2) domains recruit actin monomers for nucleation. Sca2's alpha-helical fold is unusual among Gram-negative autotransporters, which overwhelmingly fold as beta-solenoids. Rickettsia has therefore "rediscovered" formin-like actin nucleation and elongation.
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Rickettsia Sca2 has evolved formin-like activity through a different molecular mechanism.,Madasu Y, Suarez C, Kast DJ, Kovar DR, Dominguez R Proc Natl Acad Sci U S A. 2013 Jul 16;110(29):E2677-86. doi:, 10.1073/pnas.1307235110. Epub 2013 Jul 1. PMID:23818602<ref>PMID:23818602</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Large Structures]]
[[Category: Rickettsia conorii]]
[[Category: Rickettsia conorii]]
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[[Category: Dominguez, R]]
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[[Category: Dominguez R]]
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[[Category: Madasu, Y]]
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[[Category: Madasu Y]]
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[[Category: Actin]]
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[[Category: Actin nucleation]]
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[[Category: Cell invasion]]
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[[Category: Helical repeat]]
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Current revision

Structure of the N-terminal Repeat Domain of Rickettsia Sca2

PDB ID 4j7o

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