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4k37

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{{STRUCTURE_4k37| PDB=4k37 | SCENE= }}
 
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===Native anSMEcpe with bound AdoMet===
 
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{{ABSTRACT_PUBMED_23650368}}
 
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==Function==
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==Native anSMEcpe with bound AdoMet==
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[[http://www.uniprot.org/uniprot/ANSME_CLOP1 ANSME_CLOP1]] Involved in 'Cys-type' sulfatase maturation under anaerobic conditions. Catalyzes the post-translational modification of cysteine ('Cys-51' in the arylsulfatase CPF_0221) into 3-oxoalanine (also known as C(alpha)-formylglycine (FGly)), by a free radical chemical mechanism initiated via the reductive cleavage of S-adenosyl-L-methionine (SAM). Is also able to oxidize a serine residue in a synthetic substrate to FGly in vitro, and in a serine variant of a Cys-type sulfatase in vivo, but this activity is not physiological.<ref>PMID:16766528</ref> <ref>PMID:17335281</ref> <ref>PMID:18408004</ref>
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<StructureSection load='4k37' size='340' side='right'caption='[[4k37]], [[Resolution|resolution]] 1.62&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[4k37]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_perfringens_ATCC_13124 Clostridium perfringens ATCC 13124]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K37 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4K37 FirstGlance]. <br>
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[[4k37]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_perfringens_atcc_13124 Clostridium perfringens atcc 13124]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K37 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.62&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4k37 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k37 OCA], [https://pdbe.org/4k37 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4k37 RCSB], [https://www.ebi.ac.uk/pdbsum/4k37 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4k37 ProSAT]</span></td></tr>
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<ref group="xtra">PMID:023650368</ref><references group="xtra"/><references/>
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</table>
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[[Category: Clostridium perfringens atcc 13124]]
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== Function ==
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[[Category: Drennan, C L.]]
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[https://www.uniprot.org/uniprot/ANSME_CLOP1 ANSME_CLOP1] Involved in 'Cys-type' sulfatase maturation under anaerobic conditions. Catalyzes the post-translational modification of cysteine ('Cys-51' in the arylsulfatase CPF_0221) into 3-oxoalanine (also known as C(alpha)-formylglycine (FGly)), by a free radical chemical mechanism initiated via the reductive cleavage of S-adenosyl-L-methionine (SAM). Is also able to oxidize a serine residue in a synthetic substrate to FGly in vitro, and in a serine variant of a Cys-type sulfatase in vivo, but this activity is not physiological.<ref>PMID:16766528</ref> <ref>PMID:17335281</ref> <ref>PMID:18408004</ref>
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[[Category: Goldman, P J.]]
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== References ==
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[[Category: Adomet radical fold]]
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<references/>
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[[Category: Oxidoreductase]]
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__TOC__
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</StructureSection>
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[[Category: Clostridium perfringens ATCC 13124]]
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[[Category: Large Structures]]
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[[Category: Drennan CL]]
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[[Category: Goldman PJ]]

Current revision

Native anSMEcpe with bound AdoMet

PDB ID 4k37

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