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4kvg

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==Crystal structure of RIAM RA-PH domains in complex with GTP bound Rap1==
==Crystal structure of RIAM RA-PH domains in complex with GTP bound Rap1==
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<StructureSection load='4kvg' size='340' side='right' caption='[[4kvg]], [[Resolution|resolution]] 1.65&Aring;' scene=''>
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<StructureSection load='4kvg' size='340' side='right'caption='[[4kvg]], [[Resolution|resolution]] 1.65&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4kvg]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human] and [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KVG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4KVG FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4kvg]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KVG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4KVG FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene><br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.65&#8491;</td></tr>
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<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">KREV1, RAP1A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), Apbb1ip, Prel1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4kvg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kvg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4kvg RCSB], [http://www.ebi.ac.uk/pdbsum/4kvg PDBsum]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4kvg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kvg OCA], [https://pdbe.org/4kvg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4kvg RCSB], [https://www.ebi.ac.uk/pdbsum/4kvg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4kvg ProSAT]</span></td></tr>
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<table>
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</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/RAP1A_HUMAN RAP1A_HUMAN] Induces morphological reversion of a cell line transformed by a Ras oncogene. Counteracts the mitogenic function of Ras, at least partly because it can interact with Ras GAPs and RAF in a competitive manner.
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The small GTPase Rap1 induces integrin activation via an inside-out signaling pathway mediated by the Rap1-interacting adaptor molecule (RIAM). Blocking this pathway may suppress tumor metastasis and other diseases that are related to hyperactive integrins. However, the molecular basis for the specific recognition of RIAM by Rap1 remains largely unknown. Herein we present the crystal structure of an active, GTP-bound GTPase domain of Rap1 in complex with the Ras association (RA)-pleckstrin homology (PH) structural module of RIAM at 1.65 A. The structure reveals that the recognition of RIAM by Rap1 is governed by side-chain interactions. Several side chains are critical in determining specificity of this recognition, particularly the Lys31 residue in Rap1 that is oppositely charged compared with the Glu31/Asp31 residue in other Ras GTPases. Lys31 forms a salt bridge with RIAM residue Glu212, making it the key specificity determinant of the interaction. We also show that disruption of these interactions results in reduction of Rap1:RIAM association, leading to a loss of co-clustering and cell adhesion. Our findings elucidate the molecular mechanism by which RIAM mediates Rap1-induced integrin activation. The crystal structure also offers new insight into the structural basis for the specific recruitment of RA-PH module-containing effector proteins by their small GTPase partners.
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The structure of Rap1 in complex with RIAM reveals specificity determinants and recruitment mechanism.,Zhang H, Chang YC, Brennan ML, Wu J J Mol Cell Biol. 2014 Apr;6(2):128-39. doi: 10.1093/jmcb/mjt044. Epub 2013 Nov, 28. PMID:24287201<ref>PMID:24287201</ref>
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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==See Also==
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</div>
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*[[GTP-binding protein 3D structures|GTP-binding protein 3D structures]]
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Human]]
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[[Category: Homo sapiens]]
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[[Category: Lk3 transgenic mice]]
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[[Category: Large Structures]]
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[[Category: Brennan, M L.]]
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[[Category: Mus musculus]]
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[[Category: Chang, Y E.]]
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[[Category: Brennan ML]]
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[[Category: Wu, J.]]
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[[Category: Chang YE]]
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[[Category: Zhang, H.]]
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[[Category: Wu J]]
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[[Category: Actin polymerization]]
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[[Category: Zhang H]]
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[[Category: Cell adhesion]]
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[[Category: Ena/vasp]]
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[[Category: Gtpase]]
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[[Category: Integrin activation]]
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[[Category: Membrane]]
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[[Category: Mst1]]
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[[Category: Pdk]]
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[[Category: Profilin]]
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[[Category: Rap1]]
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[[Category: Rapl]]
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[[Category: Ras-related protein]]
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[[Category: Riam]]
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[[Category: Signaling protein]]
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[[Category: Ubiquitin fold]]
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Current revision

Crystal structure of RIAM RA-PH domains in complex with GTP bound Rap1

PDB ID 4kvg

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