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4m3o

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==Crystal structure of K.lactis Rtr1 NTD==
==Crystal structure of K.lactis Rtr1 NTD==
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<StructureSection load='4m3o' size='340' side='right' caption='[[4m3o]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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<StructureSection load='4m3o' size='340' side='right'caption='[[4m3o]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4m3o]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4M3O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4M3O FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4m3o]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Kluyveromyces_lactis_NRRL_Y-1140 Kluyveromyces lactis NRRL Y-1140]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4M3O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4M3O FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4m3o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4m3o OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4m3o RCSB], [http://www.ebi.ac.uk/pdbsum/4m3o PDBsum]</span></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4m3o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4m3o OCA], [https://pdbe.org/4m3o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4m3o RCSB], [https://www.ebi.ac.uk/pdbsum/4m3o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4m3o ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/Q6CK86_KLULA Q6CK86_KLULA]
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The phosphorylation state of heptapeptide repeats within the C-terminal domain (CTD) of the largest subunit of RNA polymerase II (PolII) controls the transcription cycle and is maintained by the competing action of kinases and phosphatases. Rtr1 was recently proposed to be the enzyme responsible for the transition of PolII into the elongation and termination phases of transcription by removing the phosphate marker on serine 5, but this attribution was questioned by the apparent lack of enzymatic activity. Here we demonstrate that Rtr1 is a phosphatase of new structure that is auto-inhibited by its own C-terminus. The enzymatic activity of the protein in vitro is functionally important in vivo as well: a single amino acid mutation that reduces activity leads to the same phenotype in vivo as deletion of the protein-coding gene from yeast. Surprisingly, Rtr1 dephosphorylates not only serine 5 on the CTD but also the newly described anti-termination tyrosine 1 marker, supporting the hypothesis that Rtr1 and its homologs promote the transition from transcription to termination.
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Rtr1 Is a Dual Specificity Phosphatase That Dephosphorylates Tyr1 and Ser5 on the RNA Polymerase II CTD.,Hsu PL, Yang F, Smith-Kinnaman W, Yang W, Song JE, Mosley AL, Varani G J Mol Biol. 2014 Jun 18. pii: S0022-2836(14)00303-9. doi:, 10.1016/j.jmb.2014.06.010. PMID:24951832<ref>PMID:24951832</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Hsu, P L]]
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[[Category: Kluyveromyces lactis NRRL Y-1140]]
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[[Category: Varani, G]]
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[[Category: Large Structures]]
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[[Category: Yang, W]]
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[[Category: Hsu PL]]
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[[Category: Zheng, N]]
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[[Category: Varani G]]
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[[Category: Hydrolase]]
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[[Category: Yang W]]
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[[Category: Metal binding]]
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[[Category: Zheng N]]

Current revision

Crystal structure of K.lactis Rtr1 NTD

PDB ID 4m3o

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