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4mgf

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==Crystal structure of apo-PhuS, a heme-binding protein from Pseudomonas aeruginosa==
==Crystal structure of apo-PhuS, a heme-binding protein from Pseudomonas aeruginosa==
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<StructureSection load='4mgf' size='340' side='right' caption='[[4mgf]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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<StructureSection load='4mgf' size='340' side='right'caption='[[4mgf]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4mgf]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MGF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4MGF FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4mgf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MGF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4MGF FirstGlance]. <br>
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</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4mf9|4mf9]]</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">phuS ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4mgf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mgf OCA], [https://pdbe.org/4mgf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4mgf RCSB], [https://www.ebi.ac.uk/pdbsum/4mgf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4mgf ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mgf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mgf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4mgf RCSB], [http://www.ebi.ac.uk/pdbsum/4mgf PDBsum]</span></td></tr>
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</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/O68880_PSEAI O68880_PSEAI]
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Bacterial pathogens require iron for proliferation and pathogenesis. Pseudomonas aeruginosa is a prevalent Gram-negative opportunistic human pathogen that takes advantage of immunocompromised hosts and encodes a number of proteins for uptake and utilization of iron. Here we report the crystal structures of PhuS, previously known as the cytoplasmic heme-trafficking protein from P. aeruginosa, in both the apo- and the holo-forms. In comparison to its homologue ChuS from Escherichia coli O157:H7, the heme orientation is rotated 180 degrees across the alpha-gamma axis, which may account for some of the unique functional properties of PhuS. In contrast to previous findings, heme binding does not result in an overall conformational change of PhuS. We employed spectroscopic analysis and CO measurement by gas chromatography to analyze heme degradation, demonstrating that PhuS is capable of degrading heme using ascorbic acid or cytochrome P450 reductase-NADPH as an electron donor and produces five times more CO than ChuS. Addition of catalase slows down but does not stop PhuS-catalyzed heme degradation. Through spectroscopic and mass spectrometry analysis, we identified the enzymatic product of heme degradation to be verdoheme. These data taken together suggest that PhuS is a potent heme-degrading enzyme, in addition to its proposed heme-trafficking function.
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Structural analysis and identification of PhuS as a heme-degrading enzyme from Pseudomonas aeruginosa.,Lee MJ, Schep D, McLaughlin B, Kaufmann M, Jia Z J Mol Biol. 2014 May 1;426(9):1936-46. doi: 10.1016/j.jmb.2014.02.013. Epub 2014 , Feb 20. PMID:24560694<ref>PMID:24560694</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Structural genomic]]
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[[Category: Large Structures]]
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[[Category: Jia, Z]]
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[[Category: Pseudomonas aeruginosa]]
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[[Category: Lee, M J.Y]]
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[[Category: Jia Z]]
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[[Category: Bsgi]]
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[[Category: Lee MJY]]
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[[Category: Heme]]
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[[Category: Heme-degrading enzyme]]
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[[Category: Heme-trafficking protein]]
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[[Category: Hemo]]
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[[Category: Host-pathogen interaction]]
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[[Category: Metal transport]]
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Current revision

Crystal structure of apo-PhuS, a heme-binding protein from Pseudomonas aeruginosa

PDB ID 4mgf

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