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4msx

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==Crystal structure of an essential yeast splicing factor==
==Crystal structure of an essential yeast splicing factor==
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<StructureSection load='4msx' size='340' side='right' caption='[[4msx]], [[Resolution|resolution]] 1.87&Aring;' scene=''>
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<StructureSection load='4msx' size='340' side='right'caption='[[4msx]], [[Resolution|resolution]] 1.87&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4msx]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MSX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4MSX FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4msx]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MSX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4MSX FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.87&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SAD1, YFR005C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4msx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4msx OCA], [http://pdbe.org/4msx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4msx RCSB], [http://www.ebi.ac.uk/pdbsum/4msx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4msx ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4msx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4msx OCA], [https://pdbe.org/4msx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4msx RCSB], [https://www.ebi.ac.uk/pdbsum/4msx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4msx ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/SAD1_YEAST SAD1_YEAST]] Promotes the assembly of newly synthesized U4 snRNA into the U4/U6 snRNP particle. Required for splicing of pre-mRNA.<ref>PMID:10022888</ref>
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[https://www.uniprot.org/uniprot/SAD1_YEAST SAD1_YEAST] Promotes the assembly of newly synthesized U4 snRNA into the U4/U6 snRNP particle. Required for splicing of pre-mRNA.<ref>PMID:10022888</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Sad1 is an essential splicing factor initially identified in a genetic screen in Saccharomyces cerevisiae for snRNP assembly defects. Based on sequence homology, Sad1, or USP39 in humans, is predicted to comprise two domains: a zinc finger ubiquitin binding domain (ZnF-UBP) and an inactive ubiquitin-specific protease (iUSP) domain, both of which are well conserved. The role of these domains in splicing and their interaction with ubiquitin are unknown. We first used splicing microarrays to analyze Sad1 function in vivo and found that Sad1 is critical for the splicing of nearly all yeast intron-containing genes. By using in vitro assays, we then showed that it is required for the assembly of the active spliceosome. To gain structural insights into Sad1 function, we determined the crystal structure of the full-length protein at 1.8 A resolution. In the structure, the iUSP domain forms the characteristic ubiquitin binding pocket, though with an amino acid substitution in the active site that results in complete inactivation of the enzymatic activity of the domain. The ZnF-UBP domain of Sad1 shares high structural similarly to other ZnF-UBPs; however, Sad1's ZnF-UBP does not possess the canonical ubiquitin binding motif. Given the precedents for ZnF-UBP domains to function as activators for their neighboring USP domains, we propose that Sad1's ZnF-UBP acts in a ubiquitin-independent capacity to recruit and/or activate Sad1's iUSP domain to interact with the spliceosome.
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The crystal structure of S. cerevisiae Sad1, a catalytically inactive deubiquitinase that is broadly required for pre-mRNA splicing.,Hadjivassiliou H, Rosenberg OS, Guthrie C RNA. 2014 Mar 28. PMID:24681967<ref>PMID:24681967</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4msx" style="background-color:#fffaf0;"></div>
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==See Also==
==See Also==
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*[[Pre-mRNA-splicing factor|Pre-mRNA-splicing factor]]
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*[[Pre-mRNA splicing factors 3D structures|Pre-mRNA splicing factors 3D structures]]
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Atcc 18824]]
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[[Category: Large Structures]]
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[[Category: Guthrie, C]]
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[[Category: Saccharomyces cerevisiae]]
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[[Category: Hadjivassiliou, H]]
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[[Category: Guthrie C]]
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[[Category: Rosenberg, O S]]
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[[Category: Hadjivassiliou H]]
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[[Category: Deubiquitinase]]
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[[Category: Rosenberg OS]]
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[[Category: Nuclear]]
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[[Category: Pre-mrna splicing]]
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[[Category: Pseudo-deubiquitinase]]
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[[Category: Spliceosome assembly]]
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[[Category: Splicing]]
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[[Category: Ubiquitin specific protease]]
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[[Category: Usp]]
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[[Category: Zinc finger ubiquitin binding protein]]
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[[Category: Znf-ubp]]
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Current revision

Crystal structure of an essential yeast splicing factor

PDB ID 4msx

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