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4mto

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==Structural Basis for Ca2+ Selectivity of a Voltage-gated Calcium Channel==
==Structural Basis for Ca2+ Selectivity of a Voltage-gated Calcium Channel==
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<StructureSection load='4mto' size='340' side='right' caption='[[4mto]], [[Resolution|resolution]] 3.40&Aring;' scene=''>
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<StructureSection load='4mto' size='340' side='right'caption='[[4mto]], [[Resolution|resolution]] 3.40&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4mto]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Arcb4 Arcb4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MTO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4MTO FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4mto]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Aliarcobacter_butzleri_RM4018 Aliarcobacter butzleri RM4018]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MTO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4MTO FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PX4:1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE'>PX4</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.402&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4ms2|4ms2]], [[4mtf|4mtf]], [[4mtg|4mtg]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PX4:1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE'>PX4</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Abu_1752 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=367737 ARCB4])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4mto FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mto OCA], [https://pdbe.org/4mto PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4mto RCSB], [https://www.ebi.ac.uk/pdbsum/4mto PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4mto ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mto FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mto OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4mto RCSB], [http://www.ebi.ac.uk/pdbsum/4mto PDBsum]</span></td></tr>
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</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/A8EVM5_ALIB4 A8EVM5_ALIB4]
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Voltage-gated calcium (CaV) channels catalyse rapid, highly selective influx of Ca2+ into cells despite a 70-fold higher extracellular concentration of Na+. How CaV channels solve this fundamental biophysical problem remains unclear. Here we report physiological and crystallographic analyses of a calcium selectivity filter constructed in the homotetrameric bacterial NaV channel NaVAb. Our results reveal interactions of hydrated Ca2+ with two high-affinity Ca2+-binding sites followed by a third lower-affinity site that would coordinate Ca2+ as it moves inward. At the selectivity filter entry, Site 1 is formed by four carboxyl side chains, which have a critical role in determining Ca2+ selectivity. Four carboxyls plus four backbone carbonyls form Site 2, which is targeted by the blocking cations Cd2+ and Mn2+, with single occupancy. The lower-affinity Site 3 is formed by four backbone carbonyls alone, which mediate exit into the central cavity. This pore architecture suggests a conduction pathway involving transitions between two main states with one or two hydrated Ca2+ ions bound in the selectivity filter and supports a 'knock-off' mechanism of ion permeation through a stepwise-binding process. The multi-ion selectivity filter of our CaVAb model establishes a structural framework for understanding the mechanisms of ion selectivity and conductance by vertebrate CaV channels.
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Structural basis for Ca selectivity of a voltage-gated calcium channel.,Tang L, Gamal El-Din TM, Payandeh J, Martinez GQ, Heard TM, Scheuer T, Zheng N, Catterall WA Nature. 2013 Nov 24. doi: 10.1038/nature12775. PMID:24270805<ref>PMID:24270805</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==See Also==
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</div>
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*[[Ion channels 3D structures|Ion channels 3D structures]]
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Arcb4]]
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[[Category: Aliarcobacter butzleri RM4018]]
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[[Category: Catterall, W A]]
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[[Category: Large Structures]]
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[[Category: El-Din, T M.Gamal]]
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[[Category: Catterall WA]]
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[[Category: Heard, T M]]
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[[Category: Gamal El-Din TM]]
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[[Category: Martinez, G Q]]
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[[Category: Heard TM]]
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[[Category: Payandeh, J]]
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[[Category: Martinez GQ]]
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[[Category: Scheuer, T]]
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[[Category: Payandeh J]]
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[[Category: Tang, L]]
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[[Category: Scheuer T]]
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[[Category: Zheng, N]]
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[[Category: Tang L]]
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[[Category: Calcium selective]]
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[[Category: Zheng N]]
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[[Category: Membrane]]
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[[Category: Metal transport]]
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[[Category: Tetrameric]]
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[[Category: Transport protein]]
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[[Category: Voltage-gated calcium channel]]
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[[Category: Voltage-gated ion channel]]
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Current revision

Structural Basis for Ca2+ Selectivity of a Voltage-gated Calcium Channel

PDB ID 4mto

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