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4n93

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==Alternative substrates of Mycobacterium tuberculosis anthranilate phosphoribosyl transferase==
==Alternative substrates of Mycobacterium tuberculosis anthranilate phosphoribosyl transferase==
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<StructureSection load='4n93' size='340' side='right' caption='[[4n93]], [[Resolution|resolution]] 2.03&Aring;' scene=''>
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<StructureSection load='4n93' size='340' side='right'caption='[[4n93]], [[Resolution|resolution]] 2.03&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4n93]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_tuberculosis"_(zopf_1883)_klein_1884 "bacillus tuberculosis" (zopf 1883) klein 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N93 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4N93 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4n93]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N93 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4N93 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=6M1:2-AMINO-6-METHYLBENZOIC+ACID'>6M1</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PRP:ALPHA-PHOSPHORIBOSYLPYROPHOSPHORIC+ACID'>PRP</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.03&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2bpq|2bpq]], [[3qr9|3qr9]], [[4n5v|4n5v]], [[4n8q|4n8q]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=6M1:2-AMINO-6-METHYLBENZOIC+ACID'>6M1</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PRP:ALPHA-PHOSPHORIBOSYLPYROPHOSPHORIC+ACID'>PRP</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MT2248, MTCY190.03c, Rv2192c, trpD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 "Bacillus tuberculosis" (Zopf 1883) Klein 1884])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4n93 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n93 OCA], [https://pdbe.org/4n93 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4n93 RCSB], [https://www.ebi.ac.uk/pdbsum/4n93 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4n93 ProSAT]</span></td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Anthranilate_phosphoribosyltransferase Anthranilate phosphoribosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.18 2.4.2.18] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4n93 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n93 OCA], [http://pdbe.org/4n93 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4n93 RCSB], [http://www.ebi.ac.uk/pdbsum/4n93 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4n93 ProSAT]</span></td></tr>
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</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/TRPD_MYCTU TRPD_MYCTU]
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Anthranilate phosphoribosyltransferase (AnPRT), required for the biosynthesis of tryptophan, is essential for the virulence of Mycobacterium tuberculosis (Mtb). AnPRT catalyses the Mg2+-dependent transfer of a phosphoribosyl group from 5'-phosphoribosyl 1'-pyrophosphate (PRPP) to anthranilate to form 5'-phosphoribosyl anthranilate (PRA). Mtb-AnPRT was shown to catalyse a sequential reaction and significant substrate inhibition by anthranilate was observed. Antimycobacterial fluoroanthranilates and methyl-substituted analogues were shown to act as alternative substrates for Mtb-AnPRT, producing the corresponding substituted PRA products. Structures of the enzyme complexed with anthranilate analogues reveal two distinct binding sites for anthranilate. One site is located over 8 A from PRPP at the entrance to a tunnel leading to the active site, whereas in the second, inner site anthranilate is adjacent to PRPP, in a catalytically relevant position. Soaking of the analogues for variable time periods provides evidence for anthranilate located at transient positions during transfer from the outer site to the inner catalytic site. PRPP and Mg2+ binding have been shown to be associated with the rearrangement of two flexible loops, which is required to complete the inner anthranilate binding site. It is proposed that anthranilate first binds to the outer site, providing an unusual mechanism for substrate capture and efficient transfer to the catalytic site following the binding of PRPP.
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Alternative substrates reveal catalytic cycle and key binding events in the reaction catalysed by anthranilate phosphoribosyltransferase from Mycobacterium tuberculosis.,Cookson TV, Castell A, Bulloch EM, Evans GL, Short FL, Baker EN, Lott JS, Parker EJ Biochem J. 2014 Apr 9. PMID:24712732<ref>PMID:24712732</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4n93" style="background-color:#fffaf0;"></div>
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==See Also==
==See Also==
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*[[Phosphoribosyltransferase|Phosphoribosyltransferase]]
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*[[Phosphoribosyltransferase 3D structures|Phosphoribosyltransferase 3D structures]]
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Anthranilate phosphoribosyltransferase]]
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[[Category: Large Structures]]
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[[Category: Castell, A]]
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[[Category: Mycobacterium tuberculosis]]
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[[Category: Lott, J S]]
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[[Category: Castell A]]
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[[Category: Short, F L]]
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[[Category: Lott JS]]
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[[Category: Anthranilic acid]]
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[[Category: Short FL]]
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[[Category: Inhibitor]]
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[[Category: Magnesium]]
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[[Category: Magnesium binding phosphoribosyl pyrophosphate]]
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[[Category: Transferase]]
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[[Category: Tryptophan]]
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Current revision

Alternative substrates of Mycobacterium tuberculosis anthranilate phosphoribosyl transferase

PDB ID 4n93

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