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4nss

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<StructureSection load='4nss' size='340' side='right'caption='[[4nss]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
<StructureSection load='4nss' size='340' side='right'caption='[[4nss]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4nss]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycs2 Mycs2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NSS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4NSS FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4nss]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycolicibacterium_smegmatis_MC2_155 Mycolicibacterium smegmatis MC2 155]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NSS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4NSS FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MSMEG_5817, MSMEI_5660 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=246196 MYCS2])</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4nss FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nss OCA], [http://pdbe.org/4nss PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4nss RCSB], [http://www.ebi.ac.uk/pdbsum/4nss PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4nss ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4nss FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nss OCA], [https://pdbe.org/4nss PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4nss RCSB], [https://www.ebi.ac.uk/pdbsum/4nss PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4nss ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/A0R4F7_MYCS2 A0R4F7_MYCS2]
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The success of pathogenic mycobacterial species is owing in part to their ability to parasitize the generally inhospitable phagosomal environment of host macrophages, utilizing a variety of strategies to avoid their antimycobacterial capabilities and thereby enabling their survival. A recently identified gene target in Mycobacterium smegmatis, highly conserved within Mycobacterium spp. and denoted MSMEG_5817, has been found to be important for bacterial survival within host macrophages. To gain insight into its function, the crystal structure of MSMEG_5817 has been solved to 2.40 A resolution. The structure reveals a high level of structural homology to the sterol carrier protein (SCP) family, suggesting a potential role of MSMEG_5817 in the binding and transportation of biologically relevant lipids required for bacterial survival. The lipid-binding capacity of MSMEG_5817 was confirmed by ELISA, revealing binding to a number of phospholipids with varying binding specificities compared with Homo sapiens SCP. A potential lipid-binding site was probed by alanine-scanning mutagenesis, revealing structurally relevant residues and a binding mechanism potentially differing from that of the SCPs.
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A structural and functional investigation of a novel protein from Mycobacterium smegmatis implicated in mycobacterial macrophage survivability.,Shahine A, Littler D, Brammananath R, Chan PY, Crellin PK, Coppel RL, Rossjohn J, Beddoe T Acta Crystallogr D Biol Crystallogr. 2014 Sep 1;70(Pt 9):2264-76. doi:, 10.1107/S139900471401092X. Epub 2014 Aug 23. PMID:25195741<ref>PMID:25195741</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4nss" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Mycs2]]
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[[Category: Mycolicibacterium smegmatis MC2 155]]
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[[Category: Beddoe, T]]
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[[Category: Beddoe T]]
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[[Category: Brammananath, R]]
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[[Category: Brammananath R]]
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[[Category: Chan, P Y]]
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[[Category: Chan PY]]
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[[Category: Coppel, R L]]
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[[Category: Coppel RL]]
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[[Category: Crellin, P K]]
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[[Category: Crellin PK]]
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[[Category: Littler, D]]
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[[Category: Littler D]]
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[[Category: Rossjohn, J]]
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[[Category: Rossjohn J]]
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[[Category: Shahine, A E]]
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[[Category: Shahine AE]]
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[[Category: Mycobacterial survival]]
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[[Category: Unknown function]]
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Current revision

A structural and functional investigation of a novel protein from Mycobacterium smegmatis implicated in mycobacterial macrophage survivability

PDB ID 4nss

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