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4o66

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==Crystal Structure of SMARCAL1 HARP substrate recognition domain==
==Crystal Structure of SMARCAL1 HARP substrate recognition domain==
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<StructureSection load='4o66' size='340' side='right' caption='[[4o66]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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<StructureSection load='4o66' size='340' side='right'caption='[[4o66]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4o66]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4O66 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4O66 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4o66]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4O66 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4O66 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4o66 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4o66 OCA], [http://pdbe.org/4o66 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4o66 RCSB], [http://www.ebi.ac.uk/pdbsum/4o66 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4o66 ProSAT]</span></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4o66 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4o66 OCA], [https://pdbe.org/4o66 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4o66 RCSB], [https://www.ebi.ac.uk/pdbsum/4o66 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4o66 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/SMAL1_MOUSE SMAL1_MOUSE]] ATP-dependent annealing helicase that catalyzes the rewinding of the stably unwound DNA. Rewinds single-stranded DNA bubbles that are stably bound by replication protein A (RPA). Acts throughout the genome to reanneal stably unwound DNA, performing the opposite reaction of many enzymes, such as helicases and polymerases, that unwind DNA (By similarity).
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[https://www.uniprot.org/uniprot/SMAL1_MOUSE SMAL1_MOUSE] ATP-dependent annealing helicase that catalyzes the rewinding of the stably unwound DNA. Rewinds single-stranded DNA bubbles that are stably bound by replication protein A (RPA). Acts throughout the genome to reanneal stably unwound DNA, performing the opposite reaction of many enzymes, such as helicases and polymerases, that unwind DNA (By similarity).
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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SMARCAL1, a DNA remodeling protein fundamental to genome integrity during replication, is the only gene associated with the developmental disorder Schimke immuno-osseous dysplasia (SIOD). SMARCAL1-deficient cells show collapsed replication forks, S-phase cell cycle arrest, increased chromosomal breaks, hypersensitivity to genotoxic agents, and chromosomal instability. The SMARCAL1 catalytic domain (SMARCAL1(CD)) is composed of an SNF2-type double-stranded DNA motor ATPase fused to a HARP domain of unknown function. The mechanisms by which SMARCAL1 and other DNA translocases repair replication forks are poorly understood, in part because of a lack of structural information on the domains outside of the common ATPase motor. In the present work, we determined the crystal structure of the SMARCAL1 HARP domain and examined its conformation and assembly in solution by small angle X-ray scattering. We report that this domain is conserved with the DNA mismatch and damage recognition domains of MutS/MSH and NER helicase XPB, respectively, as well as with the putative DNA specificity motif of the T4 phage fork regression protein UvsW. Loss of UvsW fork regression activity by deletion of this domain was rescued by its replacement with HARP, establishing the importance of this domain in UvsW and demonstrating a functional complementarity between these structurally homologous domains. Mutation of predicted DNA-binding residues in HARP dramatically reduced fork binding and regression activities of SMARCAL1(CD). Thus, this work has uncovered a conserved substrate recognition domain in DNA repair enzymes that couples ATP-hydrolysis to remodeling of a variety of DNA structures, and provides insight into this domain's role in replication fork stability and genome integrity.
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A structure-specific nucleic acid-binding domain conserved among DNA repair proteins.,Mason AC, Rambo RP, Greer B, Pritchett M, Tainer JA, Cortez D, Eichman BF Proc Natl Acad Sci U S A. 2014 May 27;111(21):7618-23. doi:, 10.1073/pnas.1324143111. Epub 2014 May 12. PMID:24821763<ref>PMID:24821763</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4o66" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Eichman, B F]]
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[[Category: Large Structures]]
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[[Category: Mason, A C]]
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[[Category: Mus musculus]]
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[[Category: Dna binding protein]]
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[[Category: Eichman BF]]
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[[Category: Dna repair dna replication]]
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[[Category: Mason AC]]

Current revision

Crystal Structure of SMARCAL1 HARP substrate recognition domain

PDB ID 4o66

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