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4onj

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==Crystal structure of the catalytic domain of ntDRM==
==Crystal structure of the catalytic domain of ntDRM==
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<StructureSection load='4onj' size='340' side='right' caption='[[4onj]], [[Resolution|resolution]] 2.81&Aring;' scene=''>
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<StructureSection load='4onj' size='340' side='right'caption='[[4onj]], [[Resolution|resolution]] 2.81&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4onj]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ONJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ONJ FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4onj]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Nicotiana_tabacum Nicotiana tabacum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ONJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ONJ FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SFG:SINEFUNGIN'>SFG</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.807&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4onq|4onq]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SFG:SINEFUNGIN'>SFG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4onj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4onj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4onj RCSB], [http://www.ebi.ac.uk/pdbsum/4onj PDBsum]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4onj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4onj OCA], [https://pdbe.org/4onj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4onj RCSB], [https://www.ebi.ac.uk/pdbsum/4onj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4onj ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/Q76KU6_TOBAC Q76KU6_TOBAC]
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DNA methylation is a conserved epigenetic gene-regulation mechanism. DOMAINS REARRANGED METHYLTRANSFERASE (DRM) is a key de novo methyltransferase in plants, but how DRM acts mechanistically is poorly understood. Here, we report the crystal structure of the methyltransferase domain of tobacco DRM (NtDRM) and reveal a molecular basis for its rearranged structure. NtDRM forms a functional homodimer critical for catalytic activity. We also show that Arabidopsis DRM2 exists in complex with the small interfering RNA (siRNA) effector ARGONAUTE4 (AGO4) and preferentially methylates one DNA strand, likely the strand acting as the template for RNA polymerase V-mediated noncoding RNA transcripts. This strand-biased DNA methylation is also positively correlated with strand-biased siRNA accumulation. These data suggest a model in which DRM2 is guided to target loci by AGO4-siRNA and involves base-pairing of associated siRNAs with nascent RNA transcripts.
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Molecular Mechanism of Action of Plant DRM De Novo DNA Methyltransferases.,Zhong X, Du J, Hale CJ, Gallego-Bartolome J, Feng S, Vashisht AA, Chory J, Wohlschlegel JA, Patel DJ, Jacobsen SE Cell. 2014 May 22;157(5):1050-60. doi: 10.1016/j.cell.2014.03.056. PMID:24855943<ref>PMID:24855943</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==See Also==
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</div>
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*[[DNA methyltransferase 3D structures|DNA methyltransferase 3D structures]]
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Du, J]]
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[[Category: Large Structures]]
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[[Category: Patel, D J]]
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[[Category: Nicotiana tabacum]]
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[[Category: Dna methyltransferase]]
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[[Category: Du J]]
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[[Category: Transferase-transferase inhibitor complex]]
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[[Category: Patel DJ]]

Current revision

Crystal structure of the catalytic domain of ntDRM

PDB ID 4onj

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