This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


4r2y

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (12:49, 1 March 2024) (edit) (undo)
 
(2 intermediate revisions not shown.)
Line 1: Line 1:
==Crystal structure of APC11 RING domain==
==Crystal structure of APC11 RING domain==
-
<StructureSection load='4r2y' size='340' side='right' caption='[[4r2y]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
+
<StructureSection load='4r2y' size='340' side='right'caption='[[4r2y]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
-
<table><tr><td colspan='2'>[[4r2y]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4R2Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4R2Y FirstGlance]. <br>
+
<table><tr><td colspan='2'>[[4r2y]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4R2Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4R2Y FirstGlance]. <br>
-
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.755&#8491;</td></tr>
-
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4r2y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r2y OCA], [http://pdbe.org/4r2y PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4r2y RCSB], [http://www.ebi.ac.uk/pdbsum/4r2y PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4r2y ProSAT]</span></td></tr>
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4r2y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r2y OCA], [https://pdbe.org/4r2y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4r2y RCSB], [https://www.ebi.ac.uk/pdbsum/4r2y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4r2y ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
-
[[http://www.uniprot.org/uniprot/APC11_HUMAN APC11_HUMAN]] Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. May recruit the E2 ubiquitin-conjugating enzymes to the complex.<ref>PMID:11739784</ref> <ref>PMID:18485873</ref>
+
[https://www.uniprot.org/uniprot/APC11_HUMAN APC11_HUMAN] Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. May recruit the E2 ubiquitin-conjugating enzymes to the complex.<ref>PMID:11739784</ref> <ref>PMID:18485873</ref>
-
<div style="background-color:#fffaf0;">
+
-
== Publication Abstract from PubMed ==
+
-
Polyubiquitination by E2 and E3 enzymes is a predominant mechanism regulating protein function. Some RING E3s, including anaphase-promoting complex/cyclosome (APC), catalyze polyubiquitination by sequential reactions with two different E2s. An initiating E2 ligates ubiquitin to an E3-bound substrate. Another E2 grows a polyubiquitin chain on the ubiquitin-primed substrate through poorly defined mechanisms. Here we show that human APC's RING domain is repurposed for dual functions in polyubiquitination. The canonical RING surface activates an initiating E2-ubiquitin intermediate for substrate modification. However, APC engages and activates its specialized ubiquitin chain-elongating E2 UBE2S in ways that differ from current paradigms. During chain assembly, a distinct APC11 RING surface helps deliver a substrate-linked ubiquitin to accept another ubiquitin from UBE2S. Our data define mechanisms of APC/UBE2S-mediated polyubiquitination, reveal diverse functions of RING E3s and E2s, and provide a framework for understanding distinctive RING E3 features specifying ubiquitin chain elongation.
+
-
 
+
-
Mechanism of Polyubiquitination by Human Anaphase-Promoting Complex: RING Repurposing for Ubiquitin Chain Assembly.,Brown NG, Watson ER, Weissmann F, Jarvis MA, VanderLinden R, Grace CR, Frye JJ, Qiao R, Dube P, Petzold G, Cho SE, Alsharif O, Bao J, Davidson IF, Zheng JJ, Nourse A, Kurinov I, Peters JM, Stark H, Schulman BA Mol Cell. 2014 Oct 8. pii: S1097-2765(14)00716-3. doi:, 10.1016/j.molcel.2014.09.009. PMID:25306923<ref>PMID:25306923</ref>
+
-
 
+
-
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+
-
</div>
+
-
<div class="pdbe-citations 4r2y" style="background-color:#fffaf0;"></div>
+
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
-
[[Category: Alsharif, O]]
+
[[Category: Homo sapiens]]
-
[[Category: Bao, J]]
+
[[Category: Large Structures]]
-
[[Category: Brown, N G]]
+
[[Category: Alsharif O]]
-
[[Category: Cho, S E]]
+
[[Category: Bao J]]
-
[[Category: Dube, P]]
+
[[Category: Brown NG]]
-
[[Category: Frye, J J]]
+
[[Category: Cho SE]]
-
[[Category: Grace, C R.R]]
+
[[Category: Dube P]]
-
[[Category: Jarvis, M A]]
+
[[Category: Frye JJ]]
-
[[Category: Kurinov, I]]
+
[[Category: Grace CRR]]
-
[[Category: Nourse, A]]
+
[[Category: Jarvis MA]]
-
[[Category: Peters, J M]]
+
[[Category: Kurinov I]]
-
[[Category: Petzold, G]]
+
[[Category: Nourse A]]
-
[[Category: Qiao, R]]
+
[[Category: Peters JM]]
-
[[Category: Schulman, B A]]
+
[[Category: Petzold G]]
-
[[Category: Stark, H]]
+
[[Category: Qiao R]]
-
[[Category: Vanderlinden, R]]
+
[[Category: Schulman BA]]
-
[[Category: Watson, E R]]
+
[[Category: Stark H]]
-
[[Category: Weissmann, F]]
+
[[Category: Vanderlinden R]]
-
[[Category: Zheng, J]]
+
[[Category: Watson ER]]
-
[[Category: E3 ubiquitin ligase]]
+
[[Category: Weissmann F]]
-
[[Category: Ligase]]
+
[[Category: Zheng J]]
-
[[Category: Ring domain]]
+

Current revision

Crystal structure of APC11 RING domain

PDB ID 4r2y

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4r2y"
Personal tools