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4rfs

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==Structure of a pantothenate energy coupling factor transporter==
==Structure of a pantothenate energy coupling factor transporter==
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<StructureSection load='4rfs' size='340' side='right' caption='[[4rfs]], [[Resolution|resolution]] 3.23&Aring;' scene=''>
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<StructureSection load='4rfs' size='340' side='right'caption='[[4rfs]], [[Resolution|resolution]] 3.23&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4rfs]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RFS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4RFS FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4rfs]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Levilactobacillus_brevis Levilactobacillus brevis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RFS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4RFS FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rfs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rfs OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4rfs RCSB], [http://www.ebi.ac.uk/pdbsum/4rfs PDBsum]</span></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.232&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4rfs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rfs OCA], [https://pdbe.org/4rfs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4rfs RCSB], [https://www.ebi.ac.uk/pdbsum/4rfs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4rfs ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/ECFT_LACBA ECFT_LACBA]] Transmembrane (T) component of an energy-coupling factor (ECF) ABC-transporter complex. Unlike classic ABC transporters this ECF transporter provides the energy necessary to transport a number of different substrates (By similarity). [[http://www.uniprot.org/uniprot/ECFA2_LACBA ECFA2_LACBA]] Part of a common energy-coupling factor (ECF) ABC-transporter complex. Unlike classic ABC transporters this ECF transporter provides the energy necessary to transport a number of different substrates (By similarity). [[http://www.uniprot.org/uniprot/ECFA1_LACBA ECFA1_LACBA]] Part of a common energy-coupling factor (ECF) ABC-transporter complex. Unlike classic ABC transporters this ECF transporter provides the energy necessary to transport a number of different substrates (By similarity).
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[https://www.uniprot.org/uniprot/ECFA2_LEVBA ECFA2_LEVBA] ATP-binding (A) component of a common energy-coupling factor (ECF) ABC-transporter complex. Unlike classic ABC transporters this ECF transporter provides the energy necessary to transport a number of different substrates.[HAMAP-Rule:MF_01710]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Energy-coupling factor (ECF) transporters are a unique group of ATP-binding cassette (ABC) transporters responsible for micronutrient uptake from the environment. Each ECF transporter is composed of an S component (or EcfS protein) and T/A/A' components (or EcfT/A/A' proteins; ECF module). Among the group II ECF transporters, several EcfS proteins share one ECF module; however, the underlying mechanism remains unknown. Here we report the structure of a group II ECF transporter-pantothenate transporter from Lactobacillus brevis (LbECF-PanT), which shares the ECF module with the folate and hydroxymethylpyrimidine transporters (LbECF-FolT and LbECF-HmpT). Structural and mutational analyses revealed the residues constituting the pantothenate-binding pocket. We found that although the three EcfS proteins PanT, FolT, and HmpT are dissimilar in sequence, they share a common surface area composed of the transmembrane helices 1/2/6 (SM1/2/6) to interact with the coupling helices 2/3 (CH2/3) of the same EcfT. CH2 interacts mainly with SM1 via hydrophobic interactions, which may modulate the sliding movement of EcfS. CH3 binds to a hydrophobic surface groove formed by SM1, SM2, and SM6, which may transmit the conformational changes from EcfA/A' to EcfS. We also found that the residues at the intermolecular surfaces in LbECF-PanT are essential for transporter activity, and that these residues may mediate intermolecular conformational transmission and/or affect transporter complex stability. In addition, we found that the structure of EcfT is conformationally dynamic, which supports its function as a scaffold to mediate the interaction of the ECF module with various EcfS proteins to form different transporter complexes.
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Structure of a pantothenate transporter and implications for ECF module sharing and energy coupling of group II ECF transporters.,Zhang M, Bao Z, Zhao Q, Guo H, Xu K, Wang C, Zhang P Proc Natl Acad Sci U S A. 2014 Dec 30;111(52):18560-5. doi:, 10.1073/pnas.1412246112. Epub 2014 Dec 15. PMID:25512487<ref>PMID:25512487</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Bao, Z]]
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[[Category: Large Structures]]
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[[Category: Guo, H]]
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[[Category: Levilactobacillus brevis]]
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[[Category: Xu, K]]
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[[Category: Bao Z]]
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[[Category: Zhang, M]]
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[[Category: Guo H]]
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[[Category: Zhang, P]]
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[[Category: Xu K]]
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[[Category: Zhao, Q]]
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[[Category: Zhang M]]
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[[Category: Ecf]]
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[[Category: Zhang P]]
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[[Category: Hydrolase]]
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[[Category: Zhao Q]]
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[[Category: Transport protein]]
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[[Category: Transporter]]
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Current revision

Structure of a pantothenate energy coupling factor transporter

PDB ID 4rfs

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