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4rlb

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<StructureSection load='4rlb' size='340' side='right'caption='[[4rlb]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
<StructureSection load='4rlb' size='340' side='right'caption='[[4rlb]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4rlb]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Aciba Aciba]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RLB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4RLB FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4rlb]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Acinetobacter_baumannii Acinetobacter baumannii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RLB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4RLB FirstGlance]. <br>
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</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4fuv|4fuv]], [[4rl9|4rl9]], [[4rlc|4rlc]]</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">carO ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=470 ACIBA])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4rlb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rlb OCA], [https://pdbe.org/4rlb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4rlb RCSB], [https://www.ebi.ac.uk/pdbsum/4rlb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4rlb ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rlb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rlb OCA], [http://pdbe.org/4rlb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4rlb RCSB], [http://www.ebi.ac.uk/pdbsum/4rlb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4rlb ProSAT]</span></td></tr>
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</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/Q5DL42_ACIBA Q5DL42_ACIBA]
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Outer membrane (OM) beta-barrel proteins composed of 12-18 beta-strands mediate cellular entry of small molecules in Gram-negative bacteria. Small OM proteins with barrels of 10 strands or less are not known to transport small molecules. CarO (carbapenem-associated outer membrane protein) from Acinetobacter baumannii is a small OM protein that has been implicated in the uptake of ornithine and carbapenem antibiotics. Here we report crystal structures of three isoforms of CarO. The structures are very similar and show a monomeric eight-stranded barrel lacking an open channel. CarO has a substantial extracellular domain resembling a glove that contains all the divergent residues between the different isoforms. Liposome swelling experiments demonstrate that full-length CarO and a "loop-less" truncation mutant mediate small-molecule uptake at low levels but that they are unlikely to mediate passage of carbapenem antibiotics. These results are confirmed by biased molecular dynamics simulations that allowed us to quantitatively model the transport of selected small molecules.
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Small-Molecule Transport by CarO, an Abundant Eight-Stranded beta-Barrel Outer Membrane Protein from Acinetobacterbaumannii.,Zahn M, D'Agostino T, Eren E, Basle A, Ceccarelli M, van den Berg B J Mol Biol. 2015 Apr 3. pii: S0022-2836(15)00201-6. doi:, 10.1016/j.jmb.2015.03.016. PMID:25846137<ref>PMID:25846137</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4rlb" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Aciba]]
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[[Category: Acinetobacter baumannii]]
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Basle, A]]
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[[Category: Basle A]]
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[[Category: Berg, B van den]]
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[[Category: Zahn M]]
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[[Category: Zahn, M]]
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[[Category: Van den Berg B]]
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[[Category: Beta-barrel]]
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[[Category: Membrane protein]]
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[[Category: Outer membrane protein]]
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Current revision

Crystal structure of Acinetobacter baumannii CarO2

PDB ID 4rlb

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