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4rnp

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[[Image:4rnp.jpg|left|200px]]<br /><applet load="4rnp" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="4rnp, resolution 3.0&Aring;" />
 
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'''BACTERIOPHAGE T7 RNA POLYMERASE, HIGH SALT CRYSTAL FORM, LOW TEMPERATURE DATA, ALPHA-CARBONS ONLY'''<br />
 
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==Overview==
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==BACTERIOPHAGE T7 RNA POLYMERASE, HIGH SALT CRYSTAL FORM, LOW TEMPERATURE DATA, ALPHA-CARBONS ONLY==
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The crystal structure of T7 RNA polymerase reveals a molecule organized around a cleft that can accommodate a double-stranded DNA template. A portion (approximately 45%) of the molecule displays extensive structural homology to the polymerase domain of Klenow fragment and more limited homology to the human immunodeficiency virus HIV-1 reverse transcriptase. A comparison of the structures and sequences of these polymerases identifies structural elements that may be responsible for discriminating between ribonucleotide and deoxyribonucleotide substrates, and RNA and DNA templates. The relative locations of the catalytic site and a specific promoter recognition residue allow the orientation of the polymerase on the template to be defined.
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<StructureSection load='4rnp' size='340' side='right'caption='[[4rnp]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4rnp]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_phage_T7 Escherichia phage T7]. This structure supersedes the now removed PDB entries [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3rnp 3rnp] and [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2rnp 2rnp]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RNP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4RNP FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4rnp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rnp OCA], [https://pdbe.org/4rnp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4rnp RCSB], [https://www.ebi.ac.uk/pdbsum/4rnp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4rnp ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RPOL_BPT7 RPOL_BPT7] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Responsible for the transcription of the late genes of T7. It is rifampicin-resistant. It recognizes a specific promoter sequence, unwinds the double-stranded RNA to expose the coding strand for templating, initiates transcription preferentially with a purine.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rn/4rnp_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=4rnp ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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4RNP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_t7 Bacteriophage t7]. This structure supersedes the now removed PDB entries 3RNP and 2RNP. Active as [http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RNP OCA].
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*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Crystal structure of bacteriophage T7 RNA polymerase at 3.3 A resolution., Sousa R, Chung YJ, Rose JP, Wang BC, Nature. 1993 Aug 12;364(6438):593-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7688864 7688864]
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[[Category: Escherichia phage T7]]
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[[Category: Bacteriophage t7]]
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[[Category: Large Structures]]
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[[Category: DNA-directed RNA polymerase]]
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[[Category: Liu ZJ]]
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[[Category: Single protein]]
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[[Category: Rose JP]]
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[[Category: Liu, Z J.]]
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[[Category: Sousa R]]
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[[Category: Rose, J P.]]
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[[Category: Wang BC]]
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[[Category: Sousa, R.]]
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[[Category: Wang, B C.]]
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[[Category: nucleotidyltransferase]]
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[[Category: rna polymerase]]
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[[Category: transcription]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:14:15 2008''
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Current revision

BACTERIOPHAGE T7 RNA POLYMERASE, HIGH SALT CRYSTAL FORM, LOW TEMPERATURE DATA, ALPHA-CARBONS ONLY

PDB ID 4rnp

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