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4s04

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==Crystal structure of Klebsiella pneumoniae PmrA in complex with PmrA box DNA==
==Crystal structure of Klebsiella pneumoniae PmrA in complex with PmrA box DNA==
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<StructureSection load='4s04' size='340' side='right' caption='[[4s04]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
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<StructureSection load='4s04' size='340' side='right'caption='[[4s04]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4s04]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4S04 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4S04 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4s04]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Klebsiella_pneumoniae_JM45 Klebsiella pneumoniae JM45] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4S04 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4S04 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BEF:BERYLLIUM+TRIFLUORIDE+ION'>BEF</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4s05|4s05]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BEF:BERYLLIUM+TRIFLUORIDE+ION'>BEF</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4s04 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4s04 OCA], [http://pdbe.org/4s04 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4s04 RCSB], [http://www.ebi.ac.uk/pdbsum/4s04 PDBsum]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4s04 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4s04 OCA], [https://pdbe.org/4s04 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4s04 RCSB], [https://www.ebi.ac.uk/pdbsum/4s04 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4s04 ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/A0A0R4I965_KLEPN A0A0R4I965_KLEPN]
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PmrA, an OmpR/PhoB family response regulator, manages genes for antibiotic resistance. Phosphorylation of OmpR/PhoB response regulator induces the formation of a symmetric dimer in the N-terminal receiver domain (REC), promoting two C-terminal DNA-binding domains (DBDs) to recognize promoter DNA to elicit adaptive responses. Recently, determination of the KdpE-DNA complex structure revealed an REC-DBD interface in the upstream protomer that may be necessary for transcription activation. Here, we report the 3.2-A-resolution crystal structure of the PmrA-DNA complex, which reveals a similar yet different REC-DBD interface. However, NMR studies show that in the DNA-bound state, two domains tumble separately and an REC-DBD interaction is transiently populated in solution. Reporter gene analyses of PmrA variants with altered interface residues suggest that the interface is not crucial for supporting gene expression. We propose that REC-DBD interdomain dynamics and the DBD-DBD interface help PmrA interact with RNA polymerase holoenzyme to activate downstream gene transcription.
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Structure and dynamics of polymyxin-resistance-associated response regulator PmrA in complex with promoter DNA.,Lou YC, Weng TH, Li YC, Kao YF, Lin WF, Peng HL, Chou SH, Hsiao CD, Chen C Nat Commun. 2015 Nov 13;6:8838. doi: 10.1038/ncomms9838. PMID:26564787<ref>PMID:26564787</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4s04" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Hsiao, C D]]
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[[Category: Klebsiella pneumoniae JM45]]
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[[Category: Li, Y C]]
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[[Category: Large Structures]]
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[[Category: Weng, T H]]
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[[Category: Synthetic construct]]
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[[Category: Pmra]]
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[[Category: Hsiao CD]]
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[[Category: Response regulator]]
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[[Category: Li YC]]
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[[Category: Transcription-dna complex]]
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[[Category: Weng TH]]
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[[Category: Two-component system]]
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Current revision

Crystal structure of Klebsiella pneumoniae PmrA in complex with PmrA box DNA

PDB ID 4s04

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