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4v4l

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==Structure of the Drosophila apoptosome==
==Structure of the Drosophila apoptosome==
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<StructureSection load='4v4l' size='340' side='right' caption='[[4v4l]], [[Resolution|resolution]] 6.90&Aring;' scene=''>
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<SX load='4v4l' size='340' side='right' viewer='molstar' caption='[[4v4l]], [[Resolution|resolution]] 6.90&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4v4l]] is a 16 chain structure. This structure supersedes and combines the now removed PDB entries [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1vt4 1vt4] and [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3iz8 3iz8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4V4L OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4V4L FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4v4l]] is a 16 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. This structure supersedes the now removed PDB entries [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1vt4 1vt4] and [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3iz8 3iz8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4V4L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4V4L FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DTP:2-DEOXYADENOSINE+5-TRIPHOSPHATE'>DTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 6.9&#8491;</td></tr>
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<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DTP:2-DEOXYADENOSINE+5-TRIPHOSPHATE'>DTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4v4l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4v4l OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4v4l RCSB], [http://www.ebi.ac.uk/pdbsum/4v4l PDBsum]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4v4l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4v4l OCA], [https://pdbe.org/4v4l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4v4l RCSB], [https://www.ebi.ac.uk/pdbsum/4v4l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4v4l ProSAT]</span></td></tr>
</table>
</table>
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{{Large structure}}
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== Function ==
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<div style="background-color:#fffaf0;">
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[https://www.uniprot.org/uniprot/Q7KLI1_DROME Q7KLI1_DROME]
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== Publication Abstract from PubMed ==
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The Drosophila Apaf-1 related killer forms an apoptosome in the intrinsic cell death pathway. In this study we show that Dark forms a single ring when initiator procaspases are bound. This Dark-Dronc complex cleaves DrICE efficiently; hence, a single ring represents the Drosophila apoptosome. We then determined the 3D structure of a double ring at approximately 6.9 A resolution and created a model of the apoptosome. Subunit interactions in the Dark complex are similar to those in Apaf-1 and CED-4 apoptosomes, but there are significant differences. In particular, Dark has "lost" a loop in the nucleotide-binding pocket, which opens a path for possible dATP exchange in the apoptosome. In addition, caspase recruitment domains (CARDs) form a crown on the central hub of the Dark apoptosome. This CARD geometry suggests that conformational changes will be required to form active Dark-Dronc complexes. When taken together, these data provide insights into apoptosome structure, function, and evolution.
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Structure of the Drosophila apoptosome at 6.9 a resolution.,Yuan S, Yu X, Topf M, Dorstyn L, Kumar S, Ludtke SJ, Akey CW Structure. 2011 Jan 12;19(1):128-40. PMID:21220123<ref>PMID:21220123</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==See Also==
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</div>
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*[[Cell death protein 3D structures|Cell death protein 3D structures]]
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== References ==
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<references/>
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__TOC__
__TOC__
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</StructureSection>
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</SX>
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[[Category: Akey, C W]]
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[[Category: Drosophila melanogaster]]
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[[Category: Ludtke, S J]]
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[[Category: Large Structures]]
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[[Category: Topf, M]]
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[[Category: Akey CW]]
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[[Category: Yuan, S]]
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[[Category: Ludtke SJ]]
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[[Category: Apoptosis]]
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[[Category: Topf M]]
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[[Category: Drosophila apoptosome]]
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[[Category: Yuan S]]
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[[Category: Programmed cell death]]
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Current revision

Structure of the Drosophila apoptosome

4v4l, resolution 6.90Å

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