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3pg2

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==The Crystal structure of the major pilin GBS80 of Streptococcus agalactiae 35 kDa C-terminal fragment==
==The Crystal structure of the major pilin GBS80 of Streptococcus agalactiae 35 kDa C-terminal fragment==
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<StructureSection load='3pg2' size='340' side='right' caption='[[3pg2]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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<StructureSection load='3pg2' size='340' side='right'caption='[[3pg2]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3pg2]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_agalactiae_(serotype_v) Streptococcus agalactiae (serotype v)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PG2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3PG2 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3pg2]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_agalactiae_serogroup_V Streptococcus agalactiae serogroup V]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PG2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PG2 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3pf2|3pf2]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GBS80, SAG0645 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=216466 Streptococcus agalactiae (serotype V)])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3pg2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pg2 OCA], [https://pdbe.org/3pg2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3pg2 RCSB], [https://www.ebi.ac.uk/pdbsum/3pg2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3pg2 ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3pg2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pg2 OCA], [http://pdbe.org/3pg2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3pg2 RCSB], [http://www.ebi.ac.uk/pdbsum/3pg2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3pg2 ProSAT]</span></td></tr>
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</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/Q8E0S9_STRA5 Q8E0S9_STRA5]
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The Gram-positive pathogen Streptococcus agalactiae, known as group B Streptococcus (GBS), is the leading cause of bacterial septicemia, pneumonia, and meningitis among neonates. GBS assembles two types of pili-pilus islands (PIs) 1 and 2-on its surface to adhere to host cells and to initiate colonization for pathogenesis. The GBS PI-1 pilus is made of one major pilin, GBS80, which forms the pilus shaft, and two secondary pilins, GBS104 and GBS52, which are incorporated into the pilus at various places. We report here the crystal structure of the 35-kDa C-terminal fragment from GBS80, which is composed of two IgG-like domains (N2-N3). The structure was solved by single-wavelength anomalous dispersion using sodium-iodide-soaked crystals and diffraction data collected at the home source. The N2 domain exhibits a cnaA/DEv-IgG fold with two calcium-binding sites, while the N3 domain displays a cnaB/IgG-rev fold. We have built a model for full-length GBS80 (N1, N2, and N3) with the help of available homologous major pilin structures, and we propose a model for the GBS PI-1 pilus shaft. The N2 and N3 domains are arranged in tandem along the pilus shaft, whereas the respective N1 domain is tilted by approximately 20 degrees away from the pilus axis. We have also identified a pilin-like motif in the minor pilin GBS52, which might aid its incorporation at the pilus base.
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A Model for Group B Streptococcus Pilus Type 1: The Structure of a 35-kDa C-Terminal Fragment of the Major Pilin GBS80.,Vengadesan K, Ma X, Dwivedi P, Ton-That H, Narayana SV J Mol Biol. 2011 Apr 15;407(5):731-43. Epub 2011 Feb 17. PMID:21333654<ref>PMID:21333654</ref>
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==See Also==
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*[[Pilin 3D structures|Pilin 3D structures]]
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3pg2" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Narayana, S V.L]]
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[[Category: Large Structures]]
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[[Category: Vengadesan, K]]
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[[Category: Streptococcus agalactiae serogroup V]]
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[[Category: Backbone pilin]]
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[[Category: Narayana SVL]]
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[[Category: Cell adhesion]]
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[[Category: Vengadesan K]]
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[[Category: Cna a type]]
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[[Category: Cna b type]]
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[[Category: Gb]]
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[[Category: Gbs pili]]
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[[Category: Gbs80]]
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[[Category: Group b streptococcus]]
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[[Category: Ig-like fold]]
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[[Category: Igg-dev fold]]
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[[Category: Igg-rev fold]]
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[[Category: Isopeptide bond]]
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[[Category: Isopeptide formation]]
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[[Category: Major pilin]]
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[[Category: Pilus shaft component]]
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[[Category: Structural protein]]
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Current revision

The Crystal structure of the major pilin GBS80 of Streptococcus agalactiae 35 kDa C-terminal fragment

PDB ID 3pg2

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