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3pme

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Current revision (13:32, 1 March 2024) (edit) (undo)
 
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<StructureSection load='3pme' size='340' side='right'caption='[[3pme]], [[Resolution|resolution]] 1.56&Aring;' scene=''>
<StructureSection load='3pme' size='340' side='right'caption='[[3pme]], [[Resolution|resolution]] 1.56&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3pme]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_botulinus"_van_ermengem_1896 "bacillus botulinus" van ermengem 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PME OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PME FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3pme]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PME OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PME FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.56&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">bont/C ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1491 "Bacillus botulinus" van Ermengem 1896])</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3pme FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pme OCA], [https://pdbe.org/3pme PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3pme RCSB], [https://www.ebi.ac.uk/pdbsum/3pme PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3pme ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3pme FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pme OCA], [https://pdbe.org/3pme PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3pme RCSB], [https://www.ebi.ac.uk/pdbsum/3pme PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3pme ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/Q5DW55_CLOBO Q5DW55_CLOBO]
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The botulinum neurotoxins (BoNTs) produced by different strains of the bacterium Clostridium botulinum are responsible for the disease botulism and include a group of immunologically distinct serotypes (A, B, E, and F) that are considered to be the most lethal natural proteins known for humans. Two BoNT serotypes, C and D, while rarely associated with human infection, are responsible for deadly botulism outbreaks afflicting animals. Also associated with animal infections is the BoNT C-D mosaic protein (BoNT/CD), a BoNT subtype that is essentially a hybrid of the BoNT/C ( approximately two-third) and BoNT/D ( approximately one-third) serotypes. While the amino acid sequence of the heavy chain receptor binding (HCR) domain of BoNT/CD (BoNT/CD-HCR) is very similar to the corresponding amino acid sequence of BoNT/D, BoNT/CD-HCR binds synaptosome membranes better than BoNT/D-HCR. To obtain structural insights for the different membrane binding properties, the crystal structure of BoNT/CD-HCR (S867-E1280) was determined at 1.56A resolution and compared to previously reported structures for BoNT/D-HCR. Overall, the BoNT/CD-HCR structure is similar to the two sub-domain organization observed for other BoNT HCRs: an N-terminal jellyroll barrel motif and a C-terminal beta-trefoil fold. Comparison of the structure of BoNT/CD-HCR with BoNT/D-HCR indicates that K1118 has a similar structural role as the equivalent residue, E1114, in BoNT/D-HCR, while K1136 has a structurally different role than the equivalent residue, G1132, in BoNT/D-HCR. Lysine-1118 forms a salt bridge with E1247 and may enhance membrane interactions by stabilizing the putative membrane binding loop (K1240-N1248). Lysine-1136 is observed on the surface of the protein. A sulfate ion bound to K1136 may mimic a natural interaction with the negatively changed phospholipid membrane surface. Liposome-binding experiments demonstrate that BoNT/CD-HCR binds phosphatidylethanolamine liposomes more tightly than BoNT/D-HCR.
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Crystal structure of the receptor binding domain of the botulinum C-D mosaic neurotoxin reveals potential roles of lysines 1118 and 1136 in membrane interactions.,Zhang Y, Buchko GW, Qin L, Robinson H, Varnum SM Biochem Biophys Res Commun. 2011 Jan 7;404(1):407-12. Epub 2010 Dec 3. PMID:21130733<ref>PMID:21130733</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3pme" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Bacillus botulinus van ermengem 1896]]
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[[Category: Clostridium botulinum]]
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Buchko, G W]]
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[[Category: Buchko GW]]
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[[Category: Qin, L]]
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[[Category: Qin L]]
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[[Category: Robinson, H]]
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[[Category: Robinson H]]
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[[Category: Structural genomic]]
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[[Category: Varnum SM]]
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[[Category: Varnum, S M]]
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[[Category: Zhang Y]]
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[[Category: Zhang, Y]]
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[[Category: Botulinum neurotoxin]]
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[[Category: Ganglioside]]
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[[Category: Mosaic serotype]]
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[[Category: Phosphatidylethanolamine]]
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[[Category: Receptor binding domain]]
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[[Category: Ssgcid]]
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[[Category: Toxin]]
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Current revision

Crystal structure of the receptor binding domain of botulinum neurotoxin C/D mosaic serotype

PDB ID 3pme

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