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3pqi

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== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3pqi]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacteriophage_sp. Bacteriophage sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PQI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PQI FirstGlance]. <br>
<table><tr><td colspan='2'>[[3pqi]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacteriophage_sp. Bacteriophage sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PQI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PQI FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.642&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3pqh|3pqh]]</div></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3pqi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pqi OCA], [https://pdbe.org/3pqi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3pqi RCSB], [https://www.ebi.ac.uk/pdbsum/3pqi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3pqi ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3pqi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pqi OCA], [https://pdbe.org/3pqi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3pqi RCSB], [https://www.ebi.ac.uk/pdbsum/3pqi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3pqi ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/I7HXF9_9CAUD I7HXF9_9CAUD]
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Bacteriophages with contractile tails and the bacterial type VI secretion system have been proposed to use a special protein to create an opening in the host cell membrane during infection. These proteins have a modular architecture but invariably contain an oligonucleotide/oligosaccharide-binding (OB-fold) domain and a long beta-helical C-terminal domain, which initiates the contact with the host cell membrane. Using X-ray crystallography and electron microscopy, we report the atomic structure of the membrane-piercing proteins from bacteriophages P2 and varphi92 and identify the residues that constitute the membrane-attacking apex. Both proteins form compact spikes with a approximately 10A diameter tip that is stabilized by a centrally positioned iron ion bound by six histidine residues. The accumulated data strongly suggest that, in the process of membrane penetration, the spikes are translocated through the lipid bilayer without undergoing major unfolding.
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Phage pierces the host cell membrane with the iron-loaded spike.,Browning C, Shneider MM, Bowman VD, Schwarzer D, Leiman PG Structure. 2012 Feb 8;20(2):326-39. PMID:22325780<ref>PMID:22325780</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3pqi" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Bacteriophage sp]]
[[Category: Bacteriophage sp]]
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Browning, C]]
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[[Category: Browning C]]
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[[Category: Leiman, P G]]
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[[Category: Leiman PG]]
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[[Category: Shneider, M]]
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[[Category: Shneider M]]
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[[Category: Beta-helix]]
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[[Category: Cell membrane piercing]]
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[[Category: Iron-binding]]
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[[Category: Ob-fold]]
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[[Category: Phage baseplate]]
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[[Category: Viral protein]]
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Current revision

Crystal structure of the bacteriophage phi92 membrane-piercing protein gp138

PDB ID 3pqi

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