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1mkz

From Proteopedia

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[[Image:1mkz.jpg|left|200px]]
 
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{{Structure
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==Crystal structure of MoaB protein at 1.6 A resolution.==
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|PDB= 1mkz |SIZE=350|CAPTION= <scene name='initialview01'>1mkz</scene>, resolution 1.60&Aring;
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<StructureSection load='1mkz' size='340' side='right'caption='[[1mkz]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> and <scene name='pdbligand=ACY:ACETIC ACID'>ACY</scene>
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<table><tr><td colspan='2'>[[1mkz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MKZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MKZ FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
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|GENE= MoaB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mkz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mkz OCA], [https://pdbe.org/1mkz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mkz RCSB], [https://www.ebi.ac.uk/pdbsum/1mkz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mkz ProSAT], [https://www.topsan.org/Proteins/MCSG/1mkz TOPSAN]</span></td></tr>
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</table>
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'''Crystal structure of MoaB protein at 1.6 A resolution.'''
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== Function ==
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[https://www.uniprot.org/uniprot/MOAB_ECOLI MOAB_ECOLI] May be involved in the biosynthesis of molybdopterin. Can bind GTP and has low GTPase activity. Can bind MPT, but has no MPT adenylyl transferase activity.<ref>PMID:18154309</ref>
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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The crystal structure of Escherichia coli MoaB was determined by multiwavelength anomalous diffraction phasing and refined at 1.6-A resolution. The molecule displayed a modified Rossman fold. MoaB is assembled into a hexamer composed of two trimers. The monomers have high structural similarity with two proteins, MogA and MoeA, from the molybdenum cofactor synthesis pathway in E. coli, as well as with domains of mammalian gephyrin and plant Cnx1, which are also involved in molybdopterin synthesis. Structural comparison between these proteins and the amino acid conservation patterns revealed a putative active site in MoaB. The structural analysis of this site allowed to advance several hypothesis that can be tested in further studies.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mk/1mkz_consurf.spt"</scriptWhenChecked>
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1MKZ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MKZ OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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The crystal structure of Escherichia coli MoaB suggests a probable role in molybdenum cofactor synthesis., Sanishvili R, Beasley S, Skarina T, Glesne D, Joachimiak A, Edwards A, Savchenko A, J Biol Chem. 2004 Oct 1;279(40):42139-46. Epub 2004 Jul 21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15269205 15269205]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mkz ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Edwards, A.]]
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[[Category: Edwards A]]
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[[Category: Joachimiak, A.]]
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[[Category: Joachimiak A]]
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[[Category: MCSG, Midwest Center for Structural Genomics.]]
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[[Category: Sanishvili R]]
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[[Category: Sanishvili, R.]]
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[[Category: Savchenko A]]
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[[Category: Savchenko, A.]]
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[[Category: Skarina T]]
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[[Category: Skarina, T.]]
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[[Category: ACY]]
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[[Category: SO4]]
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[[Category: mad]]
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[[Category: mcsg]]
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[[Category: midwest center for structural genomic]]
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[[Category: molybdopterin synthesis]]
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[[Category: protein structure initiative]]
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[[Category: psi]]
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[[Category: structural genomic]]
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[[Category: weak anomalous signal]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:44:46 2008''
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Current revision

Crystal structure of MoaB protein at 1.6 A resolution.

PDB ID 1mkz

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