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4z2y

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==Crystal structure of methyltransferase CalO6==
==Crystal structure of methyltransferase CalO6==
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<StructureSection load='4z2y' size='340' side='right' caption='[[4z2y]], [[Resolution|resolution]] 3.40&Aring;' scene=''>
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<StructureSection load='4z2y' size='340' side='right'caption='[[4z2y]], [[Resolution|resolution]] 3.40&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4z2y]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Z2Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Z2Y FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4z2y]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Micromonospora_echinospora Micromonospora echinospora]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Z2Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4Z2Y FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.4&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4z2y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4z2y OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4z2y RCSB], [http://www.ebi.ac.uk/pdbsum/4z2y PDBsum]</span></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4z2y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4z2y OCA], [https://pdbe.org/4z2y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4z2y RCSB], [https://www.ebi.ac.uk/pdbsum/4z2y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4z2y ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/Q8KND2_MICEC Q8KND2_MICEC]
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BACKGROUND: Calicheamicins (CAL) are enedyine natural products with potent antibiotic and cytotoxic activity, used in anticancer therapy. The O-methyltransferase CalO6 is proposed to catalyze methylation of the hydroxyl moiety at the C2 position of the orsellinic acid group of CAL. RESULTS: Crystals of CalO6 diffracted non-isotropically, with the usable data extending to 3.4 A. While no single method of crystal structure determination yielded a structure of CalO6, we were able to determine its structure by using molecular replacement-guided single wavelength anomalous dispersion by using diffraction data from native crystals of CalO6 and a highly non-isomorphous mercury derivative. The structure of CalO6 reveals the methyltransferase fold and dimeric organization characteristic of small molecule O-methyltransferases involved in secondary metabolism in bacteria and plants. Uncommonly, CalO6 was crystallized in the absence of S-adenosylmethionine (SAM; the methyl donor) or S-adenosylhomocysteine (SAH; its product). CONCLUSIONS: Likely as a consequence of the dynamic nature of CalO6 in the absence of its cofactor, the central region of CalO6, which forms a helical lid-like structure near the active site in CalO6 and similar enzymes, is not observed in the electron density. We propose that this region controls the entry of SAM into and the exit of SAH from the active site of CalO6 and shapes the active site for substrate binding and catalysis.
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Crystal structure of O-methyltransferase CalO6 from the calicheamicin biosynthetic pathway: a case of challenging structure determination at low resolution.,Tsodikov OV, Hou C, Walsh CT, Garneau-Tsodikova S BMC Struct Biol. 2015 Jul 15;15:13. doi: 10.1186/s12900-015-0040-6. PMID:26170207<ref>PMID:26170207</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Garneau-Tsodikova, S]]
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[[Category: Large Structures]]
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[[Category: Hou, C]]
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[[Category: Micromonospora echinospora]]
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[[Category: Tsodikov, O V]]
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[[Category: Garneau-Tsodikova S]]
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[[Category: Antibiotic]]
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[[Category: Hou C]]
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[[Category: Anticancer]]
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[[Category: Tsodikov OV]]
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[[Category: Calicheamicin]]
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[[Category: O-methyltransferase]]
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[[Category: Transferase]]
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Current revision

Crystal structure of methyltransferase CalO6

PDB ID 4z2y

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