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5cgz

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Crystal structure of GalB, the 4-carboxy-2-hydroxymuconate hydratase, from Pseuodomonas putida KT2440

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Retrieved from "http://52.214.119.220/wiki/index.php/5cgz"

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 <StructureSection load='5cgz' size='340' side='right'caption='[[5cgz]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5cgz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Psepk Psepk]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CGZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5CGZ FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5cgz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_putida_KT2440 Pseudomonas putida KT2440]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CGZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5CGZ FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.103&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">galB, PP_2515 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=160488 PSEPK])</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/4-oxalmesaconate_hydratase 4-oxalmesaconate hydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.83 4.2.1.83] </span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5cgz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cgz OCA], [https://pdbe.org/5cgz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5cgz RCSB], [https://www.ebi.ac.uk/pdbsum/5cgz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5cgz ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5cgz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cgz OCA], [http://pdbe.org/5cgz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5cgz RCSB], [http://www.ebi.ac.uk/pdbsum/5cgz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5cgz ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/GALB_PSEPK GALB_PSEPK]] Catalyzes the conversion of oxalomesaconic acid enol (OMAenol) to 4-carboxy-4-hydroxy-2-oxoadipic acid (CHA). Mediates the third step of gallate degradation pathway.<ref>PMID:21219457</ref>
+[https://www.uniprot.org/uniprot/GALB_PSEPK GALB_PSEPK] Catalyzes the conversion of oxalomesaconic acid enol (OMAenol) to 4-carboxy-4-hydroxy-2-oxoadipic acid (CHA). Mediates the third step of gallate degradation pathway.<ref>PMID:21219457</ref>
-<div style="background-color:#fffaf0;">
-== Publication Abstract from PubMed ==
-Bacterial catabolism of lignin and its breakdown products is of interest for applications in industrial processing of ligno-biomass. The gallate degradation pathway of Pseudomonas putida KT2440 requires a 4-carboxy-2-hydroxymuconate (CHM) hydratase (GalB) which has 12% sequence identity to a previously identified CHM hydratase (LigJ) from Sphingomonas sp. SYK-6. The structure of GalB was determined and found to be a member of the PIG-L N-acetyl glucosamine deacetylase family, and is structurally distinct from the amidohydrolase fold of LigJ. LigJ has the same stereo-specificity as GalB, providing an example of convergent evolution for catalytic conversion of a common metabolite in bacterial aromatic degradation pathways. Purified GalB contained bound Zn2+ cofactor; however the enzyme is capable using Fe2+ and Co2+ with similar efficiencies. The general base aspartate in the PIG-L deacetylases is an alanine in GalB; replacement of the alanine with aspartate decreased the GalB catalytic efficiency for CHM by 9.5 x 104 fold and the variant enzyme did not have any detectable hydrolase activity. Kinetic analyses and pH dependency studies of the wild-type and variant enzymes suggests roles for the Glu48 and His164 in the catalytic mechanism. Comparison to the PIG-L deacetylases has led to a proposed mechanism for GalB wherein Glu48 positions and activates the metal ligated water for the hydration reaction and His164 acts as a catalytic acid.
-Structural and Kinetic Characterization of the 4-Carboxy-2-Hydroxymuconate Hydratase from the Gallate and Protocatechuate 4,5-Cleavage Pathways of Pseudomonas putida KT2440.,Mazurkewich S, Brott AS, Kimber MS, Seah SY J Biol Chem. 2016 Feb 11. pii: jbc.M115.682054. PMID:26867578<ref>PMID:26867578</ref>
-From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-</div>
-<div class="pdbe-citations 5cgz" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: 4-oxalmesaconate hydratase]]
 [[Category: Large Structures]]
-[[Category: Psepk]]
+[[Category: Pseudomonas putida KT2440]]
-[[Category: Brott, A S]]
+[[Category: Brott AS]]
-[[Category: Kimber, M S]]
+[[Category: Kimber MS]]
-[[Category: Mazurkewich, S]]
+[[Category: Mazurkewich S]]
-[[Category: Seah, S Y.K]]
+[[Category: Seah SYK]]
-[[Category: Galb]]
-[[Category: Gallic acid]]
-[[Category: Helical hairpin]]
-[[Category: Hexamer]]
-[[Category: Hydratase]]
-[[Category: Lyase]]
-[[Category: Metalloenzyme]]
-[[Category: Rossmann fold]]

5cgz

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Crystal structure of GalB, the 4-carboxy-2-hydroxymuconate hydratase, from Pseuodomonas putida KT2440

Structural highlights

Function

References

Contents

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