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5cuw
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 5cuw is ON HOLD Authors: Kattke, M.D., Cascio, D., Sawaya, M.R., Clubb, R.T. Description: Crystal structure of Sortase E1 from Streptomyces coelico...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of Sortase E1 from Streptomyces coelicolor with tripeptide in the active site== | |
| - | + | <StructureSection load='5cuw' size='340' side='right'caption='[[5cuw]], [[Resolution|resolution]] 1.89Å' scene=''> | |
| - | + | == Structural highlights == | |
| - | + | <table><tr><td colspan='2'>[[5cuw]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_coelicolor_A3(2) Streptomyces coelicolor A3(2)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CUW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5CUW FirstGlance]. <br> | |
| - | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.887Å</td></tr> | |
| - | [[Category: | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> |
| - | [[Category: | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5cuw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cuw OCA], [https://pdbe.org/5cuw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5cuw RCSB], [https://www.ebi.ac.uk/pdbsum/5cuw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5cuw ProSAT]</span></td></tr> |
| - | [[Category: | + | </table> |
| - | [[Category: | + | == Function == |
| - | [[Category: | + | [https://www.uniprot.org/uniprot/SRTE1_STRCO SRTE1_STRCO] Transpeptidase that anchors surface proteins to the cell wall. Recognizes both Leu-Ala-x-Thr-Gly and Leu-Pro-x-Thr-Gly, with a preference for the former. Unlike the S.aureus sortase it cleaves not only the Thr-Gly motif but also the Ala-X bond; Ala-Glu and Ala-His bonds are better substrates than the Thr-Gly motif in vitro (PubMed:22296345, PubMed:27936128). Among its possible substrates are the chaplins ChpA, ChpB and ChpC; this enzyme is less important for ChpC attachment than is SrtE2. A double knockout mutant of srtE1 and srtE2 shows a developmental defect in aerial hyphae formation more dramatic than that due to chaplin deletion (PubMed:22296345).<ref>PMID:22296345</ref> <ref>PMID:27936128</ref> |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Cascio D]] | ||
| + | [[Category: Clubb RT]] | ||
| + | [[Category: Kattke MD]] | ||
| + | [[Category: Sawaya MR]] | ||
Current revision
Crystal structure of Sortase E1 from Streptomyces coelicolor with tripeptide in the active site
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