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5hax

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==Crystal structure of Chaetomium thermophilum Nup170 NTD-Nup53 complex==
==Crystal structure of Chaetomium thermophilum Nup170 NTD-Nup53 complex==
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<StructureSection load='5hax' size='340' side='right' caption='[[5hax]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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<StructureSection load='5hax' size='340' side='right'caption='[[5hax]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5hax]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HAX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5HAX FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5hax]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Chaetomium_thermophilum_var._thermophilum_DSM_1495 Chaetomium thermophilum var. thermophilum DSM 1495]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HAX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5HAX FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5hay|5hay]], [[5haz|5haz]], [[5hb2|5hb2]], [[5hb1|5hb1]], [[5hb0|5hb0]], [[5hb3|5hb3]], [[5hb4|5hb4]], [[5hb5|5hb5]], [[5hb6|5hb6]], [[5hb7|5hb7]], [[5hb8|5hb8]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5hax FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hax OCA], [http://pdbe.org/5hax PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hax RCSB], [http://www.ebi.ac.uk/pdbsum/5hax PDBsum]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5hax FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hax OCA], [https://pdbe.org/5hax PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5hax RCSB], [https://www.ebi.ac.uk/pdbsum/5hax PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5hax ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/NUP53_CHATD NUP53_CHATD]
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The nuclear pore complex (NPC) controls the transport of macromolecules between the nucleus and cytoplasm, but its molecular architecture has thus far remained poorly defined. We biochemically reconstituted NPC core protomers and elucidated the underlying protein-protein interaction network. Flexible linker sequences, rather than interactions between the structured core scaffold nucleoporins, mediate the assembly of the inner ring complex and its attachment to the NPC coat. X-ray crystallographic analysis of these scaffold nucleoporins revealed the molecular details of their interactions with the flexible linker sequences and enabled construction of full-length atomic structures. By docking these structures into the cryoelectron tomographic reconstruction of the intact human NPC and validating their placement with our nucleoporin interactome, we built a composite structure of the NPC symmetric core that contains ~320,000 residues and accounts for ~56 megadaltons of the NPC's structured mass. Our approach provides a paradigm for the structure determination of similarly complex macromolecular assemblies.
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Architecture of the symmetric core of the nuclear pore.,Lin DH, Stuwe T, Schilbach S, Rundlet EJ, Perriches T, Mobbs G, Fan Y, Thierbach K, Huber FM, Collins LN, Davenport AM, Jeon YE, Hoelz A Science. 2016 Apr 15;352(6283):aaf1015. doi: 10.1126/science.aaf1015. Epub 2016, Apr 14. PMID:27081075<ref>PMID:27081075</ref>
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==See Also==
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*[[Nucleoporin 3D structures|Nucleoporin 3D structures]]
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 5hax" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Hoelz, A]]
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[[Category: Chaetomium thermophilum var. thermophilum DSM 1495]]
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[[Category: Lin, D H]]
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[[Category: Large Structures]]
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[[Category: Mobbs, G]]
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[[Category: Hoelz A]]
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[[Category: Nucleocytoplasmic transport]]
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[[Category: Lin DH]]
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[[Category: Protein transport]]
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[[Category: Mobbs G]]
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[[Category: Transport protein]]
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Current revision

Crystal structure of Chaetomium thermophilum Nup170 NTD-Nup53 complex

PDB ID 5hax

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