This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


5iou

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: ==Cryo-EM structure of GluN1/GluN2B NMDA receptor in the glutamate/glycine-bound conformation== <StructureSection load='5iou' size='340' side='right' caption='5iou, [[Resolution|resol...)
Current revision (12:34, 6 March 2024) (edit) (undo)
 
(5 intermediate revisions not shown.)
Line 1: Line 1:
==Cryo-EM structure of GluN1/GluN2B NMDA receptor in the glutamate/glycine-bound conformation==
==Cryo-EM structure of GluN1/GluN2B NMDA receptor in the glutamate/glycine-bound conformation==
-
<StructureSection load='5iou' size='340' side='right' caption='[[5iou]], [[Resolution|resolution]] 7.00&Aring;' scene=''>
+
<SX load='5iou' size='340' side='right' viewer='molstar' caption='[[5iou]], [[Resolution|resolution]] 7.00&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
-
<table><tr><td colspan='2'>[[5iou]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IOU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5IOU FirstGlance]. <br>
+
<table><tr><td colspan='2'>[[5iou]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IOU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5IOU FirstGlance]. <br>
-
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GLU:GLUTAMIC+ACID'>GLU</scene>, <scene name='pdbligand=GLY:GLYCINE'>GLY</scene></td></tr>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 7&#8491;</td></tr>
-
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5iov|5iov]], [[5ipq|5ipq]], [[5ips|5ips]], [[5ipr|5ipr]], [[5ipt|5ipt]], [[5ipu|5ipu]], [[5ipv|5ipv]]</td></tr>
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLU:GLUTAMIC+ACID'>GLU</scene>, <scene name='pdbligand=GLY:GLYCINE'>GLY</scene></td></tr>
-
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5iou FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5iou OCA], [http://pdbe.org/5iou PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5iou RCSB], [http://www.ebi.ac.uk/pdbsum/5iou PDBsum]</span></td></tr>
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5iou FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5iou OCA], [https://pdbe.org/5iou PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5iou RCSB], [https://www.ebi.ac.uk/pdbsum/5iou PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5iou ProSAT]</span></td></tr>
</table>
</table>
-
<div style="background-color:#fffaf0;">
+
== Function ==
-
== Publication Abstract from PubMed ==
+
[https://www.uniprot.org/uniprot/NMDZ1_XENLA NMDZ1_XENLA] Component of NMDA receptor complexes that function as heterotetrameric, ligand-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Channel activation requires binding of the neurotransmitter glutamate to the epsilon subunit, glycine binding to the zeta subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+) (PubMed:16214956, PubMed:19524674, PubMed:21677647, PubMed:25008524, PubMed:26912815, PubMed:27135925, Ref.11, PubMed:28232581). Sensitivity to glutamate and channel kinetics depend on the subunit composition (Probable).<ref>PMID:16214956</ref> <ref>PMID:19524674</ref> <ref>PMID:21677647</ref> <ref>PMID:25008524</ref> <ref>PMID:26912815</ref> <ref>PMID:27135925</ref> <ref>PMID:28232581</ref> [PDB:5IOV]
-
N-methyl-D-aspartate receptors (NMDARs) are glutamate-gated, calcium-permeable ion channels that mediate synaptic transmission and underpin learning and memory. NMDAR dysfunction is directly implicated in diseases ranging from seizure to ischemia. Despite its fundamental importance, little is known about how the NMDAR transitions between inactive and active states and how small molecules inhibit or activate ion channel gating. Here, we report electron cryo-microscopy structures of the GluN1-GluN2B NMDA receptor in an ensemble of competitive antagonist-bound states, an agonist-bound form, and a state bound with agonists and the allosteric inhibitor Ro25-6981. Together with double electron-electron resonance experiments, we show how competitive antagonists rupture the ligand binding domain (LBD) gating "ring," how agonists retain the ring in a dimer-of-dimers configuration, and how allosteric inhibitors, acting within the amino terminal domain, further stabilize the LBD layer. These studies illuminate how the LBD gating ring is fundamental to signal transduction and gating in NMDARs.
+
-
Mechanism of NMDA Receptor Inhibition and Activation.,Zhu S, Stein RA, Yoshioka C, Lee CH, Goehring A, Mchaourab HS, Gouaux E Cell. 2016 Apr 21;165(3):704-14. doi: 10.1016/j.cell.2016.03.028. Epub 2016 Apr, 7. PMID:27062927<ref>PMID:27062927</ref>
+
==See Also==
-
 
+
*[[Glutamate receptor 3D structures|Glutamate receptor 3D structures]]
-
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+
-
</div>
+
-
<div class="pdbe-citations 5iou" style="background-color:#fffaf0;"></div>
+
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
-
</StructureSection>
+
</SX>
-
[[Category: Goehring, A]]
+
[[Category: Large Structures]]
-
[[Category: Gouaux, E]]
+
[[Category: Xenopus laevis]]
-
[[Category: Lee, C H]]
+
[[Category: Goehring A]]
-
[[Category: Mchaourab, S H]]
+
[[Category: Gouaux E]]
-
[[Category: Stein, A R]]
+
[[Category: Lee CH]]
-
[[Category: Yoshioka, C]]
+
[[Category: Mchaourab SH]]
-
[[Category: Zhu, S]]
+
[[Category: Stein AR]]
-
[[Category: Ligand-gated ion channel]]
+
[[Category: Yoshioka C]]
-
[[Category: Signaling protein]]
+
[[Category: Zhu S]]
-
[[Category: Synaptic transmission]]
+

Current revision

Cryo-EM structure of GluN1/GluN2B NMDA receptor in the glutamate/glycine-bound conformation

5iou, resolution 7.00Å

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5iou"
Personal tools