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5jm7

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==The structure of aerobactin synthetase IucA from a hypervirulent pathotype of Klebsiella pneumoniae==
==The structure of aerobactin synthetase IucA from a hypervirulent pathotype of Klebsiella pneumoniae==
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<StructureSection load='5jm7' size='340' side='right' caption='[[5jm7]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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<StructureSection load='5jm7' size='340' side='right'caption='[[5jm7]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5jm7]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JM7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5JM7 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5jm7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Klebsiella_pneumoniae_subsp._pneumoniae Klebsiella pneumoniae subsp. pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JM7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5JM7 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5jm8|5jm8]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5jm7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jm7 OCA], [http://pdbe.org/5jm7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5jm7 RCSB], [http://www.ebi.ac.uk/pdbsum/5jm7 PDBsum]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5jm7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jm7 OCA], [https://pdbe.org/5jm7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5jm7 RCSB], [https://www.ebi.ac.uk/pdbsum/5jm7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5jm7 ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/A0A1A9TA96_KLEPN A0A1A9TA96_KLEPN]
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Iron is a vital mineral nutrient required by virtually all life forms to prosper; pathogenic bacteria are no exception. Despite the abundance of iron within the human host, highly regulated iron physiology can result in exceedingly low levels of iron bioavailable to prospective invading bacteria. To combat this scarcity of iron, many pathogenic bacteria have acquired specific and efficient iron acquisition systems, which allow them to thrive in iron-deficient host environments. One of the more prominent bacterial iron acquisition systems involves the synthesis, secretion, and reuptake of small-molecule iron chelators known as siderophores. Aerobactin, a citrate-hydroxamate siderophore originally isolated nearly 50 years ago, is produced by a number of pathogenic Gram-negative bacteria. Aerobactin has recently been demonstrated to play a pivotal role in mediating the enhanced virulence of a particularly invasive pathotype of Klebsiella pneumoniae (hvKP). Toward further understanding of this key virulence factor, we report the structural and functional characterization of aerobactin synthetase IucA from a strain of hvKP. The X-ray crystal structures of unliganded and ATP-bound forms of IucA were solved, forming the foundation of our structural analysis. Small angle X-ray scattering (SAXS) data suggest that, unlike its closest structurally characterized homologues, IucA adopts a tetrameric assembly in solution. Finally, we employed activity assays to investigate the substrate specificity and determine the apparent steady-state kinetic parameters of IucA.
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Structural and Functional Characterization of Aerobactin Synthetase IucA from a Hypervirulent Pathotype of Klebsiella pneumoniae.,Bailey DC, Drake EJ, Grant TD, Gulick AM Biochemistry. 2016 Jun 16. PMID:27253399<ref>PMID:27253399</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 5jm7" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Bailey, D C]]
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[[Category: Klebsiella pneumoniae subsp. pneumoniae]]
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[[Category: Drake, E J]]
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[[Category: Large Structures]]
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[[Category: Gulick, A M]]
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[[Category: Bailey DC]]
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[[Category: Aerobactin nis synthetase]]
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[[Category: Drake EJ]]
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[[Category: Ligase]]
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[[Category: Gulick AM]]

Current revision

The structure of aerobactin synthetase IucA from a hypervirulent pathotype of Klebsiella pneumoniae

PDB ID 5jm7

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