5k7c

<StructureSection load='5k7c' size='340' side='right' caption='[[5k7c]], [[Resolution|resolution]] 2.73&Aring;' scene=''>

<table><tr><td colspan='2'>[[5k7c]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5K7C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5K7C FirstGlance]. <br>

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>

<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5k7d|5k7d]], [[5k7e|5k7e]]</td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5k7c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5k7c OCA], [http://pdbe.org/5k7c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5k7c RCSB], [http://www.ebi.ac.uk/pdbsum/5k7c PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5k7c ProSAT]</span></td></tr>

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== Publication Abstract from PubMed ==

The field of small self-cleaving nucleolytic ribozymes has been invigorated by the recent discovery of the twister, twister-sister, pistol and hatchet ribozymes. We report the crystal structure of a pistol ribozyme termed env25, which adopts a compact tertiary architecture stabilized by an embedded pseudoknot fold. The G-U cleavage site adopts a splayed-apart conformation with in-line alignment of the modeled 2'-O of G for attack on the adjacent to-be-cleaved P-O5' bond. Highly conserved residues G40 (N1 position) and A32 (N3 and 2'-OH positions) are aligned to act as a general base and a general acid, respectively, to accelerate cleavage chemistry, with their roles confirmed by cleavage assays on variants, and an increased pKa of 4.7 for A32. Our structure of the pistol ribozyme defined how the overall and local topologies dictate the in-line alignment at the G-U cleavage site, with cleavage assays on variants revealing key residues that participate in acid-base-catalyzed cleavage chemistry.

Pistol ribozyme adopts a pseudoknot fold facilitating site-specific in-line cleavage.,Ren A, Vusurovic N, Gebetsberger J, Gao P, Juen M, Kreutz C, Micura R, Patel DJ Nat Chem Biol. 2016 Jul 11. doi: 10.1038/nchembio.2125. PMID:27398999<ref>PMID:27398999</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>

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<div class="pdbe-citations 5k7c" style="background-color:#fffaf0;"></div>

*[[Ribozyme|Ribozyme]]

[[Category: Patel, D]]

[[Category: Ren, A]]

[[Category: Pistol]]

[[Category: Ribozyme]]

[[Category: Rna-dna complex]]