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| | <StructureSection load='5kev' size='340' side='right'caption='[[5kev]], [[Resolution|resolution]] 2.70Å' scene=''> | | <StructureSection load='5kev' size='340' side='right'caption='[[5kev]], [[Resolution|resolution]] 2.70Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5kev]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Vibpa Vibpa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KEV OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5KEV FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5kev]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_parahaemolyticus_RIMD_2210633 Vibrio parahaemolyticus RIMD 2210633]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KEV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5KEV FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5kew|5kew]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">VPA1332 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=223926 VIBPA]), VPA1333 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=223926 VIBPA])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5kev FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kev OCA], [https://pdbe.org/5kev PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5kev RCSB], [https://www.ebi.ac.uk/pdbsum/5kev PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5kev ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5kev FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kev OCA], [http://pdbe.org/5kev PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5kev RCSB], [http://www.ebi.ac.uk/pdbsum/5kev PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5kev ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/Q87GI4_VIBPA Q87GI4_VIBPA] |
| - | Bile is an important component of the human gastrointestinal tract with an essential role in food absorption and antimicrobial activities. Enteric bacterial pathogens have developed strategies to sense bile as an environmental cue to regulate virulence genes during infection. We discovered that Vibrio parahaemolyticus VtrC, along with VtrA and VtrB, are required for activating the virulence type III secretion system 2 in response to bile salts. The VtrA/VtrC complex activates VtrB in the presence of bile salts. The crystal structure of the periplasmic domains of the VtrA/VtrC heterodimer reveals a beta-barrel with a hydrophobic inner chamber. A co-crystal structure of VtrA/VtrC with bile salt, along with biophysical and mutational analysis, demonstrates that the hydrophobic chamber binds bile salts and activates the virulence network. As part of a family of conserved signaling receptors, VtrA/VtrC provides structural and functional insights into the evolutionarily conserved mechanism used by bacteria to sense their environment.
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| - | | + | |
| - | Bile salt receptor complex activates a pathogenic type III secretion system.,Li P, Rivera-Cancel G, Kinch LN, Salomon D, Tomchick DR, Grishin NV, Orth K Elife. 2016 Jul 5;5. pii: e15718. doi: 10.7554/eLife.15718. PMID:27377244<ref>PMID:27377244</ref>
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| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 5kev" style="background-color:#fffaf0;"></div>
| + | |
| - | == References ==
| + | |
| - | <references/>
| + | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Vibpa]] | + | [[Category: Vibrio parahaemolyticus RIMD 2210633]] |
| - | [[Category: Orth, K]] | + | [[Category: Orth K]] |
| - | [[Category: Rivera-Cancel, G]] | + | [[Category: Rivera-Cancel G]] |
| - | [[Category: Tomchick, D R]] | + | [[Category: Tomchick DR]] |
| - | [[Category: Alpha/beta]]
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| - | [[Category: Bile salt receptor]]
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| - | [[Category: Calycin beta barrel superfamily]]
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| - | [[Category: Heterodimer]]
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| - | [[Category: Signaling protein]]
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| - | [[Category: Transcription]]
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