5khs

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<StructureSection load='5khs' size='340' side='right'caption='[[5khs]], [[Resolution|resolution]] 3.76&Aring;' scene=''>
<StructureSection load='5khs' size='340' side='right'caption='[[5khs]], [[Resolution|resolution]] 3.76&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5khs]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KHS OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5KHS FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5khs]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Burkholderia_multivorans Burkholderia multivorans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KHS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5KHS FirstGlance]. <br>
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</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5khn|5khn]]</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.758&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5khs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5khs OCA], [http://pdbe.org/5khs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5khs RCSB], [http://www.ebi.ac.uk/pdbsum/5khs PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5khs ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5khs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5khs OCA], [https://pdbe.org/5khs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5khs RCSB], [https://www.ebi.ac.uk/pdbsum/5khs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5khs ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/HPNN_BURM1 HPNN_BURM1] Essential for hopanoid transport from the cytoplasmic to the outer membrane (PubMed:28584102). Is capable of shuttling hopanoid lipids from the inner membrane to the periplasm, where they probably spontaneously insert to the inner leaflet of the outer membrane, strengthening the cell envelope (PubMed:28584102). May be a proton-motive-force (PMF)-dependent transporter (PubMed:28584102). Is critical for multidrug resistance and cell wall remodeling in Burkholderia (PubMed:28584102).<ref>PMID:28584102</ref>
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Strains of the Burkholderia cepacia complex (Bcc) are Gram-negative opportunisitic bacteria that are capable of causing serious diseases, mainly in immunocompromised individuals. Bcc pathogens are intrinsically resistant to multiple antibiotics, including beta-lactams, aminoglycosides, fluoroquinolones, and polymyxins. They are major pathogens in patients with cystic fibrosis (CF) and can cause severe necrotizing pneumonia, which is often fatal. Hopanoid biosynthesis is one of the major mechanisms involved in multiple antimicrobial resistance of Bcc pathogens. The hpnN gene of B. multivorans encodes an integral membrane protein of the HpnN family of transporters, which is responsible for shuttling hopanoids to the outer membrane. Here, we report crystal structures of B. multivorans HpnN, revealing a dimeric molecule with an overall butterfly shape. Each subunit of the transporter contains 12 transmembrane helices and two periplasmic loops that suggest a plausible pathway for substrate transport. Further analyses indicate that HpnN is capable of shuttling hopanoid virulence factors from the outer leaflet of the inner membrane to the periplasm. Taken together, our data suggest that the HpnN transporter is critical for multidrug resistance and cell wall remodeling in Burkholderia.
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Crystal structures of the Burkholderia multivorans hopanoid transporter HpnN.,Kumar N, Su CC, Chou TH, Radhakrishnan A, Delmar JA, Rajashankar KR, Yu EW Proc Natl Acad Sci U S A. 2017 Jun 20;114(25):6557-6562. doi:, 10.1073/pnas.1619660114. Epub 2017 Jun 5. PMID:28584102<ref>PMID:28584102</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 5khs" style="background-color:#fffaf0;"></div>
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== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Burkholderia multivorans]]
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Su, C C]]
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[[Category: Su C-C]]
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[[Category: Yu, E W]]
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[[Category: Yu EW]]
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[[Category: Membrane protein]]
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[[Category: Transmembrane helice]]
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Current revision

Crystal structures of the Burkholderia multivorans hopanoid transporter HpnN

PDB ID 5khs

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