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5pfs

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==PanDDA analysis group deposition -- Crystal Structure of BAZ2B after initial refinement with no ligand modelled (structure 156)==
==PanDDA analysis group deposition -- Crystal Structure of BAZ2B after initial refinement with no ligand modelled (structure 156)==
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<StructureSection load='5pfs' size='340' side='right' caption='[[5pfs]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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<StructureSection load='5pfs' size='340' side='right'caption='[[5pfs]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5pfs]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5PFS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5PFS FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5pfs]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5PFS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5PFS FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5pfs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5pfs OCA], [http://pdbe.org/5pfs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5pfs RCSB], [http://www.ebi.ac.uk/pdbsum/5pfs PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5pfs ProSAT]</span></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5pfs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5pfs OCA], [https://pdbe.org/5pfs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5pfs RCSB], [https://www.ebi.ac.uk/pdbsum/5pfs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5pfs ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/BAZ2B_HUMAN BAZ2B_HUMAN]] May play a role in transcriptional regulation interacting with ISWI.
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[https://www.uniprot.org/uniprot/BAZ2B_HUMAN BAZ2B_HUMAN] May play a role in transcriptional regulation interacting with ISWI.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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In macromolecular crystallography, the rigorous detection of changed states (for example, ligand binding) is difficult unless signal is strong. Ambiguous ('weak' or 'noisy') density is experimentally common, since molecular states are generally only fractionally present in the crystal. Existing methodologies focus on generating maximally accurate maps whereby minor states become discernible; in practice, such map interpretation is disappointingly subjective, time-consuming and methodologically unsound. Here we report the PanDDA method, which automatically reveals clear electron density for the changed state-even from inaccurate maps-by subtracting a proportion of the confounding 'ground state'; changed states are objectively identified from statistical analysis of density distributions. The method is completely general, implying new best practice for all changed-state studies, including the routine collection of multiple ground-state crystals. More generally, these results demonstrate: the incompleteness of atomic models; that single data sets contain insufficient information to model them fully; and that accuracy requires further map-deconvolution approaches.
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A multi-crystal method for extracting obscured crystallographic states from conventionally uninterpretable electron density.,Pearce NM, Krojer T, Bradley AR, Collins P, Nowak RP, Talon R, Marsden BD, Kelm S, Shi J, Deane CM, von Delft F Nat Commun. 2017 Apr 24;8:15123. doi: 10.1038/ncomms15123. PMID:28436492<ref>PMID:28436492</ref>
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==See Also==
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*[[Bromodomain adjacent to zinc finger 3D structures|Bromodomain adjacent to zinc finger 3D structures]]
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 5pfs" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Arrowsmith, C H]]
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[[Category: Homo sapiens]]
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[[Category: Bountra, C]]
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[[Category: Large Structures]]
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[[Category: Bradley, A R]]
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[[Category: Arrowsmith CH]]
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[[Category: Brandao-Neto, J]]
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[[Category: Bountra C]]
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[[Category: Brennan, P E]]
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[[Category: Bradley AR]]
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[[Category: Collins, P]]
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[[Category: Brandao-Neto J]]
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[[Category: Cox, O]]
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[[Category: Brennan PE]]
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[[Category: Delft, F von]]
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[[Category: Collins P]]
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[[Category: Dias, A]]
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[[Category: Cox O]]
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[[Category: Douangamath, A]]
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[[Category: Dias A]]
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[[Category: Edwards, A]]
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[[Category: Douangamath A]]
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[[Category: Fairhead, M]]
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[[Category: Edwards A]]
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[[Category: Krojer, T]]
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[[Category: Fairhead M]]
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[[Category: MacLean, E]]
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[[Category: Krojer T]]
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[[Category: Ng, J]]
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[[Category: MacLean E]]
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[[Category: Pearce, N M]]
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[[Category: Ng J]]
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[[Category: Renjie, Z]]
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[[Category: Pearce NM]]
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[[Category: Sethi, R]]
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[[Category: Renjie Z]]
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[[Category: Talon, R]]
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[[Category: Sethi R]]
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[[Category: Vollmar, M]]
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[[Category: Talon R]]
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[[Category: Wright, N]]
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[[Category: Vollmar M]]
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[[Category: Bromodomain]]
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[[Category: Wright N]]
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[[Category: Dna binding protein]]
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[[Category: Von Delft F]]
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[[Category: Epigenetic]]
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[[Category: Pandda]]
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[[Category: Sgc - diamond i04-1 fragment screening]]
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Current revision

PanDDA analysis group deposition -- Crystal Structure of BAZ2B after initial refinement with no ligand modelled (structure 156)

PDB ID 5pfs

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