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5t59

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Current revision (14:19, 6 March 2024) (edit) (undo)
 
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<StructureSection load='5t59' size='340' side='right'caption='[[5t59]], [[Resolution|resolution]] 2.41&Aring;' scene=''>
<StructureSection load='5t59' size='340' side='right'caption='[[5t59]], [[Resolution|resolution]] 2.41&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5t59]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5T59 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5T59 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5t59]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Kluyveromyces_lactis_NRRL_Y-1140 Kluyveromyces lactis NRRL Y-1140]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5T59 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5T59 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NHE:2-[N-CYCLOHEXYLAMINO]ETHANE+SULFONIC+ACID'>NHE</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.405&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5t51|5t51]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NHE:2-[N-CYCLOHEXYLAMINO]ETHANE+SULFONIC+ACID'>NHE</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5t59 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5t59 OCA], [http://pdbe.org/5t59 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5t59 RCSB], [http://www.ebi.ac.uk/pdbsum/5t59 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5t59 ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5t59 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5t59 OCA], [https://pdbe.org/5t59 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5t59 RCSB], [https://www.ebi.ac.uk/pdbsum/5t59 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5t59 ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/Q6CLK3_KLULA Q6CLK3_KLULA]
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Kinetochores connect centromeric nucleosomes with mitotic-spindle microtubules through conserved, cross-interacting protein subassemblies. In budding yeast, the heterotetrameric MIND complex (Mtw1, Nnf1, Nsl1, Dsn1), ortholog of the metazoan Mis12 complex, joins the centromere-proximal components, Mif2 and COMA, with the principal microtubule-binding component, the Ndc80 complex (Ndc80C). We report the crystal structure of Kluyveromyces lactis MIND and examine its partner interactions, to understand the connection from a centromeric nucleosome to a much larger microtubule. MIND resembles an elongated, asymmetric Y; two globular heads project from a coiled-coil shaft. An N-terminal extension of Dsn1 from one head regulates interactions of the other head, blocking binding of Mif2 and COMA. Dsn1 phosphorylation by Ipl1/Aurora B relieves this autoinhibition, enabling MIND to join an assembling kinetochore. A C-terminal extension of Dsn1 recruits Ndc80C to the opposite end of the shaft. The structure and properties of MIND show how it integrates phospho-regulatory inputs for kinetochore assembly and disassembly.
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Structure of the MIND Complex Defines a Regulatory Focus for Yeast Kinetochore Assembly.,Dimitrova YN, Jenni S, Valverde R, Khin Y, Harrison SC Cell. 2016 Nov 3;167(4):1014-1027.e12. doi: 10.1016/j.cell.2016.10.011. Epub 2016, Oct 27. PMID:27881300<ref>PMID:27881300</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 5t59" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Kluyveromyces lactis NRRL Y-1140]]
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Dimitrova, Y]]
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[[Category: Dimitrova Y]]
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[[Category: Harrison, S C]]
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[[Category: Harrison SC]]
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[[Category: Jenni, S]]
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[[Category: Jenni S]]
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[[Category: Khin, Y]]
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[[Category: Khin Y]]
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[[Category: Valverde, R]]
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[[Category: Valverde R]]
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[[Category: Cell cycle]]
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[[Category: Chromosome]]
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[[Category: Complex]]
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[[Category: Kinetochore]]
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[[Category: Mind]]
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[[Category: Mis12]]
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[[Category: Segregation]]
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Current revision

Structure of the MIND Complex Shows a Regulatory Focus of Yeast Kinetochore Assembly

PDB ID 5t59

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