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5u22

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Current revision (14:24, 6 March 2024) (edit) (undo)
 
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<StructureSection load='5u22' size='340' side='right'caption='[[5u22]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
<StructureSection load='5u22' size='340' side='right'caption='[[5u22]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5u22]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Neofr Neofr]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5U22 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5U22 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5u22]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Neocallimastix_frontalis Neocallimastix frontalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5U22 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5U22 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BTB:2-[BIS-(2-HYDROXY-ETHYL)-AMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>BTB</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.748&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5u22 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5u22 OCA], [http://pdbe.org/5u22 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5u22 RCSB], [http://www.ebi.ac.uk/pdbsum/5u22 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5u22 ProSAT]</span></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BTB:2-[BIS-(2-HYDROXY-ETHYL)-AMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>BTB</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5u22 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5u22 OCA], [https://pdbe.org/5u22 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5u22 RCSB], [https://www.ebi.ac.uk/pdbsum/5u22 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5u22 ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/A0A247D6Y4_NEOFR A0A247D6Y4_NEOFR]
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Enzyme activities that improve digestion of recalcitrant plant cell wall polysaccharides may offer solutions for sustainable industries. To this end, anaerobic fungi in the rumen have been identified as a promising source of novel carbohydrate active enzymes (CAZymes) that modify plant cell wall polysaccharides and other complex glycans. Many CAZymes share insufficient sequence identity to characterized proteins from other microbial ecosystems to infer their function; thus presenting challenges to their identification. In this study, four rumen fungal genes (nf2152, nf2215, nf2523, and pr2455) were identified that encode family 39 glycoside hydrolases (GH39s), and have conserved structural features with GH51s. Two recombinant proteins, NF2152 and NF2523, were characterized using a variety of biochemical and structural techniques, and were determined to have distinct catalytic activities. NF2152 releases a single product, beta1,2-arabinobiose (Ara2) from sugar beet arabinan (SBA), and beta1,2-Ara2 and alpha-1,2-galactoarabinose (Gal-Ara) from rye arabinoxylan (RAX). NF2523 exclusively releases alpha-1,2-Gal-Ara from RAX, which represents the first description of a galacto-(alpha-1,2)-arabinosidase. Both beta-1,2-Ara2 and alpha-1,2-Gal-Ara are disaccharides not previously described within SBA and RAX. In this regard, the enzymes studied here may represent valuable new biocatalytic tools for investigating the structures of rare arabinosyl-containing glycans, and potentially for facilitating their modification in industrial applications.
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Discovery and characterization of family 39 glycoside hydrolases from rumen anaerobic fungi with polyspecific activity on rare arabinosyl substrates.,Jones DR, Uddin MS, Gruninger RJ, Pham TTM, Thomas D, Boraston AB, Briggs J, Pluvinage B, McAllister TA, Forster RJ, Tsang A, Selinger LB, Abbott DW J Biol Chem. 2017 Jul 28;292(30):12606-12620. doi: 10.1074/jbc.M117.789008. Epub , 2017 Jun 6. PMID:28588026<ref>PMID:28588026</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 5u22" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Neofr]]
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[[Category: Neocallimastix frontalis]]
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[[Category: Abbott, D W]]
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[[Category: Abbott DW]]
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[[Category: Jones, D R]]
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[[Category: Jones DR]]
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[[Category: Beta sandwich]]
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[[Category: Glycoside hydrolase]]
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[[Category: Hydrolase]]
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[[Category: Tim barrel]]
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Current revision

Structure of N2152 from Neocallimastix frontalis

PDB ID 5u22

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