This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


6xpd

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (14:51, 6 March 2024) (edit) (undo)
 
Line 3: Line 3:
<StructureSection load='6xpd' size='340' side='right'caption='[[6xpd]], [[Resolution|resolution]] 3.80&Aring;' scene=''>
<StructureSection load='6xpd' size='340' side='right'caption='[[6xpd]], [[Resolution|resolution]] 3.80&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
-
<table><tr><td colspan='2'>[[6xpd]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XPD OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6XPD FirstGlance]. <br>
+
<table><tr><td colspan='2'>[[6xpd]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XPD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6XPD FirstGlance]. <br>
-
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.8&#8491;</td></tr>
-
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SLC30A8, ZNT8 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6xpd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xpd OCA], [http://pdbe.org/6xpd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6xpd RCSB], [http://www.ebi.ac.uk/pdbsum/6xpd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6xpd ProSAT]</span></td></tr>
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xpd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xpd OCA], [https://pdbe.org/6xpd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xpd RCSB], [https://www.ebi.ac.uk/pdbsum/6xpd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xpd ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
-
[[http://www.uniprot.org/uniprot/ZNT8_HUMAN ZNT8_HUMAN]] Facilitates the accumulation of zinc from the cytoplasm into intracellular vesicles, being a zinc-efflux transporter. May be a major component for providing zinc to insulin maturation and/or storage processes in insulin-secreting pancreatic beta-cells.<ref>PMID:16984975</ref>
+
[https://www.uniprot.org/uniprot/ZNT8_HUMAN ZNT8_HUMAN] Facilitates the accumulation of zinc from the cytoplasm into intracellular vesicles, being a zinc-efflux transporter. May be a major component for providing zinc to insulin maturation and/or storage processes in insulin-secreting pancreatic beta-cells.<ref>PMID:16984975</ref>
-
<div style="background-color:#fffaf0;">
+
-
== Publication Abstract from PubMed ==
+
-
ZnT8 is a Zn(2+)/H(+) antiporter that belongs to SLC30 family and plays an essential role in regulating Zn(2+) accumulation in the insulin secretory granules of pancreatic beta cells. Dysfunction of ZnT8 is associated with both type 1 and 2 diabetes. However, the Zn(2+)/H(+) exchange mechanism of ZnT8 remains unclear due to the lack of high-resolution structures. Here, we report the cryo-EM structures of human ZnT8 (HsZnT8) in both outward- and inward-facing conformations. HsZnT8 forms a dimeric structure with four Zn(2+) binding sites within each subunit: a highly conserved primary site in transmembrane domain (TMD) housing the Zn(2+) substrate; an interfacial site between TMD and C-terminal domain (CTD) that modulates the Zn(2+) transport activity of HsZnT8; and two adjacent sites buried in the cytosolic domain and chelated by conserved residues from CTD and the His-Cys-His (HCH) motif from the N-terminal segment of the neighboring subunit. A comparison of the outward- and inward-facing structures reveals that the TMD of each HsZnT8 subunit undergoes a large structural rearrangement, allowing for alternating access to the primary Zn(2+) site during the transport cycle. Collectively, our studies provide the structural insights into the Zn(2+)/H(+) exchange mechanism of HsZnT8.
+
-
 
+
-
Cryo-EM structures of human ZnT8 in both outward- and inward-facing conformations.,Xue J, Xie T, Zeng W, Jiang Y, Bai XC Elife. 2020 Jul 29;9. pii: 58823. doi: 10.7554/eLife.58823. PMID:32723473<ref>PMID:32723473</ref>
+
-
 
+
-
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+
-
</div>
+
-
<div class="pdbe-citations 6xpd" style="background-color:#fffaf0;"></div>
+
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
-
[[Category: Human]]
+
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Large Structures]]
-
[[Category: Bai, X C]]
+
[[Category: Bai XC]]
-
[[Category: Jiang, Y X]]
+
[[Category: Jiang YX]]
-
[[Category: Xue, J]]
+
[[Category: Xue J]]
-
[[Category: Transport protein]]
+
-
[[Category: Zinc transporter]]
+
-
[[Category: Znt8]]
+

Current revision

Cryo-EM structure of human ZnT8 double mutant - D110N and D224N, determined in outward-facing conformation

PDB ID 6xpd

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6xpd"
Personal tools