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7lfw

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Current revision (14:59, 6 March 2024) (edit) (undo)
 
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<StructureSection load='7lfw' size='340' side='right'caption='[[7lfw]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
<StructureSection load='7lfw' size='340' side='right'caption='[[7lfw]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[7lfw]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7LFW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7LFW FirstGlance]. <br>
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<table><tr><td colspan='2'>[[7lfw]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7LFW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7LFW FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PCG:CYCLIC+GUANOSINE+MONOPHOSPHATE'>PCG</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CNGA1, CNCG, CNCG1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PCG:CYCLIC+GUANOSINE+MONOPHOSPHATE'>PCG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7lfw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7lfw OCA], [https://pdbe.org/7lfw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7lfw RCSB], [https://www.ebi.ac.uk/pdbsum/7lfw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7lfw ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7lfw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7lfw OCA], [https://pdbe.org/7lfw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7lfw RCSB], [https://www.ebi.ac.uk/pdbsum/7lfw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7lfw ProSAT]</span></td></tr>
</table>
</table>
== Disease ==
== Disease ==
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[[https://www.uniprot.org/uniprot/CNGA1_HUMAN CNGA1_HUMAN]] Retinitis pigmentosa. The disease is caused by variants affecting the gene represented in this entry.
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[https://www.uniprot.org/uniprot/CNGA1_HUMAN CNGA1_HUMAN] Retinitis pigmentosa. The disease is caused by variants affecting the gene represented in this entry.
== Function ==
== Function ==
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[[https://www.uniprot.org/uniprot/CNGA1_HUMAN CNGA1_HUMAN]] Subunit of the rod cyclic GMP-gated cation channel, which is involved in the final stage of the phototransduction pathway. When light hits rod photoreceptors, cGMP concentrations decrease causing rapid closure of CNGA1/CNGB1 channels and, therefore, hyperpolarization of the membrane potential.[UniProtKB:Q00194]
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[https://www.uniprot.org/uniprot/CNGA1_HUMAN CNGA1_HUMAN] Subunit of the rod cyclic GMP-gated cation channel, which is involved in the final stage of the phototransduction pathway. When light hits rod photoreceptors, cGMP concentrations decrease causing rapid closure of CNGA1/CNGB1 channels and, therefore, hyperpolarization of the membrane potential.[UniProtKB:Q00194]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Mammalian cyclic nucleotide-gated (CNG) channels play an essential role in the signal transduction of the visual and olfactory sensory systems. Here we reveal the structural mechanism of ligand gating in human rod CNGA1 channel by determining its cryo-EM structures in both the apo closed and cGMP-bound open states. Distinct from most other members of voltage-gated tetrameric cation channels, CNGA1 forms a central channel gate in the middle of the membrane, occluding the central cavity. Structural analyses of ion binding profiles in the selectivity filters of the wild-type channel and the E365Q filter mutant allow us to unambiguously define the two Ca(2+) binding sites inside the selectivity filter, providing structural insights into Ca(2+) blockage and permeation in CNG channels. The structure of the E365Q mutant also reveals two alternative side-chain conformations at Q365, providing a plausible explanation for the voltage-dependent gating of CNG channel acquired upon E365 mutation.
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Structural mechanisms of gating and selectivity of human rod CNGA1 channel.,Xue J, Han Y, Zeng W, Wang Y, Jiang Y Neuron. 2021 Feb 19. pii: S0896-6273(21)00081-7. doi:, 10.1016/j.neuron.2021.02.007. PMID:33651975<ref>PMID:33651975</ref>
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==See Also==
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*[[Ion channels 3D structures|Ion channels 3D structures]]
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 7lfw" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Human]]
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[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Han, Y]]
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[[Category: Han Y]]
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[[Category: Jiang, Y]]
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[[Category: Jiang Y]]
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[[Category: Xue, J]]
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[[Category: Xue J]]
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[[Category: Alpha-helical]]
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[[Category: Ion channel]]
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[[Category: Membrane protein]]
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Current revision

Cryo-EM structure of human cGMP-bound open CNGA1 channel in K+/Ca2+

PDB ID 7lfw

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