This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


5t17

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (15:34, 6 March 2024) (edit) (undo)
 
(2 intermediate revisions not shown.)
Line 1: Line 1:
==NMR structure of the E. coli protein NPr, residues 1-85==
==NMR structure of the E. coli protein NPr, residues 1-85==
-
<StructureSection load='5t17' size='340' side='right' caption='[[5t17]], [[NMR_Ensembles_of_Models | 30 NMR models]]' scene=''>
+
<StructureSection load='5t17' size='340' side='right'caption='[[5t17]]' scene=''>
== Structural highlights ==
== Structural highlights ==
-
<table><tr><td colspan='2'>[[5t17]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5T17 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5T17 FirstGlance]. <br>
+
<table><tr><td colspan='2'>[[5t17]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_O157:H7 Escherichia coli O157:H7]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5T17 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5T17 FirstGlance]. <br>
-
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5t17 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5t17 OCA], [http://pdbe.org/5t17 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5t17 RCSB], [http://www.ebi.ac.uk/pdbsum/5t17 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5t17 ProSAT]</span></td></tr>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5t17 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5t17 OCA], [https://pdbe.org/5t17 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5t17 RCSB], [https://www.ebi.ac.uk/pdbsum/5t17 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5t17 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
-
[[http://www.uniprot.org/uniprot/PTSO_ECO57 PTSO_ECO57]] Component of the phosphoenolpyruvate-dependent nitrogen-metabolic phosphotransferase system (nitrogen-metabolic PTS), that seems to be involved in regulating nitrogen metabolism. The phosphoryl group from phosphoenolpyruvate (PEP) is transferred to the phosphoryl carrier protein NPr by enzyme I-Ntr. Phospho-NPr then transfers it to EIIA-Ntr. Could function in the transcriptional regulation of sigma-54 dependent operons in conjunction with the NPr (PtsO) and EIIA-Ntr (PtsN) proteins (By similarity).
+
[https://www.uniprot.org/uniprot/PTSO_ECOLI PTSO_ECOLI] Component of the phosphoenolpyruvate-dependent nitrogen-metabolic phosphotransferase system (nitrogen-metabolic PTS), that seems to be involved in regulating nitrogen metabolism. The phosphoryl group from phosphoenolpyruvate (PEP) is transferred to the phosphoryl carrier protein NPr by enzyme I-Ntr. Phospho-NPr then transfers it to EIIA-Ntr. Could function in the transcriptional regulation of sigma-54 dependent operons in conjunction with the NPr (PtsO) and EIIA-Ntr (PtsN) proteins.
-
<div style="background-color:#fffaf0;">
+
-
== Publication Abstract from PubMed ==
+
-
A nitrogen-related signal transduction pathway, consisting of the three phosphotransfer proteins EI(Ntr), NPr, and IIA(Ntr), was discovered recently to regulate the uptake of K(+) in Escherichia coli. In particular, dephosphorylated IIA(Ntr) inhibits the activity of the K(+) transporter TrkA. Since the phosphorylation state of IIA(Ntr) is partially determined by its reversible phosphorylation by NPr, we have determined the three-dimensional structure of NPr by solution NMR spectroscopy. In total, we obtained 973 NOE-derived distance restraints, 112 chemical shift-derived backbone angle restraints, and 35 hydrogen-bond restraints derived from temperature coefficients (wave). We propose that temperature wave is useful for identifying exposed beta-strands and assists in establishing protein folds based on chemical shifts. The deduced structure of NPr contains three alpha-helices and four beta-strands with the three helices all packed on the same face of the beta-sheet. The active site residue His16 of NPr for phosphoryl transfer was found to be neutral and in the N epsilon 2-H tautomeric state. There appears to be increased motion in the active site region of NPr compared to HPr, a homologous protein involved in the uptake and regulation of carbohydrate utilization.
+
-
Solution structure of NPr, a bacterial signal-transducing protein that controls the phosphorylation state of the potassium transporter-regulating protein IIA Ntr.,Li X, Peterkofsky A, Wang G Amino Acids. 2008 Oct;35(3):531-9. doi: 10.1007/s00726-008-0079-9. Epub 2008 Apr , 18. PMID:18421563<ref>PMID:18421563</ref>
+
==See Also==
-
 
+
*[[Phosphocarrier protein HPr 3D structures|Phosphocarrier protein HPr 3D structures]]
-
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+
-
</div>
+
-
<div class="pdbe-citations 5t17" style="background-color:#fffaf0;"></div>
+
-
== References ==
+
-
<references/>
+
__TOC__
__TOC__
</StructureSection>
</StructureSection>
-
[[Category: Li, X]]
+
[[Category: Escherichia coli O157:H7]]
-
[[Category: Peterkofsky, A]]
+
[[Category: Large Structures]]
-
[[Category: Wang, G]]
+
[[Category: Li X]]
-
[[Category: Biofilm]]
+
[[Category: Peterkofsky A]]
-
[[Category: Hpr-like]]
+
[[Category: Wang G]]
-
[[Category: Phosphotransfer protein]]
+
-
[[Category: Ptsntr]]
+
-
[[Category: Transferase]]
+

Current revision

NMR structure of the E. coli protein NPr, residues 1-85

PDB ID 5t17

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5t17"
Personal tools