This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


5t5p

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (15:36, 6 March 2024) (edit) (undo)
 
(One intermediate revision not shown.)
Line 1: Line 1:
==LECTIN FROM BAUHINIA FORFICATA IN COMPLEX WITH TN-PEPTIDE==
==LECTIN FROM BAUHINIA FORFICATA IN COMPLEX WITH TN-PEPTIDE==
-
<StructureSection load='5t5p' size='340' side='right' caption='[[5t5p]], [[Resolution|resolution]] 1.66&Aring;' scene=''>
+
<StructureSection load='5t5p' size='340' side='right'caption='[[5t5p]], [[Resolution|resolution]] 1.66&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
-
<table><tr><td colspan='2'>[[5t5p]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Baufo Baufo]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5T5P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5T5P FirstGlance]. <br>
+
<table><tr><td colspan='2'>[[5t5p]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bauhinia_forficata Bauhinia forficata] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5T5P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5T5P FirstGlance]. <br>
-
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=A2G:N-ACETYL-2-DEOXY-2-AMINO-GALACTOSE'>A2G</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.66&#8491;</td></tr>
-
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene></td></tr>
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A2G:N-ACETYL-2-DEOXY-2-AMINO-GALACTOSE'>A2G</scene>, <scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
-
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5t50|5t50]], [[5t52|5t52]], [[5t55|5t55]], [[5t5l|5t5l]], [[5t5o|5t5o]], [[5t54|5t54]], [[5t5j|5t5j]]</td></tr>
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5t5p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5t5p OCA], [https://pdbe.org/5t5p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5t5p RCSB], [https://www.ebi.ac.uk/pdbsum/5t5p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5t5p ProSAT]</span></td></tr>
-
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5t5p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5t5p OCA], [http://pdbe.org/5t5p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5t5p RCSB], [http://www.ebi.ac.uk/pdbsum/5t5p PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5t5p ProSAT]</span></td></tr>
+
</table>
</table>
-
<div style="background-color:#fffaf0;">
+
== Function ==
-
== Publication Abstract from PubMed ==
+
[https://www.uniprot.org/uniprot/LECT_BAUFO LECT_BAUFO]
-
Lectins have been used at length for basic research and clinical applications. New insights into the molecular recognition properties enhance our basic understanding of carbohydrate-protein interactions and aid in the design/development of new lectins. In this study, we used a combination of cell-based assays, glycan microarrays, and X-ray crystallography to evaluate the structure and function of the recombinant Bauhinia forficata lectin (BfL). The lectin was shown to be cytostatic for several cancer cell lines included in the NCI-60 panel; in particular, it inhibited growth of melanoma cancer cells (LOX IMVI) by over 95%. BfL is dimeric in solution and highly specific for binding of oligosaccharides and glycopeptides with terminal N-acetylgalactosamine (GalNAc). BfL was found to have especially strong binding (apparent Kd = 0.5-1.0 nm) to the tumor-associated Tn antigen. High-resolution crystal structures were determined for the ligand-free lectin, as well as for its complexes with three Tn glycopeptides, globotetraose, and the blood group A antigen. Extensive analysis of the eight crystal structures and comparison to structures of related lectins revealed several unique features of GalNAc recognition. Of special note, the carboxylate group of Glu126, lining the glycan-binding pocket, forms H-bonds with both the N-acetyl of GalNAc and the peptide amido group of Tn antigens. Stabilization provided by Glu126 is described here for the first time for any GalNAc-specific lectin. Taken together, the results provide new insights into the molecular recognition of carbohydrates and provide a structural understanding that will enable rational engineering of BfL for a variety of applications. DATABASE: Structural data are available in the PDB under the accession numbers 5T50, 5T52, 5T55, 5T54, 5T5L, 5T5J, 5T5P, and 5T5O.
+
-
 
+
-
Structural analysis and unique molecular recognition properties of a Bauhinia forficata lectin that inhibits cancer cell growth.,Lubkowski J, Durbin SV, Silva MC, Farnsworth D, Gildersleeve JC, Oliva ML, Wlodawer A FEBS J. 2017 Feb;284(3):429-450. doi: 10.1111/febs.13989. Epub 2017 Feb 1. PMID:27973758<ref>PMID:27973758</ref>
+
-
 
+
-
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+
-
</div>
+
-
<div class="pdbe-citations 5t5p" style="background-color:#fffaf0;"></div>
+
-
== References ==
+
-
<references/>
+
__TOC__
__TOC__
</StructureSection>
</StructureSection>
-
[[Category: Baufo]]
+
[[Category: Bauhinia forficata]]
-
[[Category: Lubkowski, J]]
+
[[Category: Homo sapiens]]
-
[[Category: Wlodawer, A]]
+
[[Category: Large Structures]]
-
[[Category: Concanavalin some]]
+
[[Category: Lubkowski J]]
-
[[Category: Galnac-specific]]
+
[[Category: Wlodawer A]]
-
[[Category: Legume lectin]]
+
-
[[Category: Ligand-free]]
+
-
[[Category: Sugar binding protein]]
+

Current revision

LECTIN FROM BAUHINIA FORFICATA IN COMPLEX WITH TN-PEPTIDE

PDB ID 5t5p

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5t5p"
Personal tools