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| | <SX load='5tcp' size='340' side='right' viewer='molstar' caption='[[5tcp]], [[Resolution|resolution]] 4.30Å' scene=''> | | <SX load='5tcp' size='340' side='right' viewer='molstar' caption='[[5tcp]], [[Resolution|resolution]] 4.30Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5tcp]] is a 48 chain structure with sequence from [http://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_typhimurium Salmonella enterica subsp. enterica serovar typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TCP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5TCP FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5tcp]] is a 48 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium Salmonella enterica subsp. enterica serovar Typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TCP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5TCP FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5tcq|5tcq]], [[5tcr|5tcr]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.3Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5tcp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5tcp OCA], [http://pdbe.org/5tcp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5tcp RCSB], [http://www.ebi.ac.uk/pdbsum/5tcp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5tcp ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5tcp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5tcp OCA], [https://pdbe.org/5tcp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5tcp RCSB], [https://www.ebi.ac.uk/pdbsum/5tcp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5tcp ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/PRGK_SALTY PRGK_SALTY]] Required for invasion of epithelial cells. Could be involved in protein secretion. [[http://www.uniprot.org/uniprot/PRGH_SALTY PRGH_SALTY]] Required for invasion of epithelial cells. | + | [https://www.uniprot.org/uniprot/PRGK_SALTY PRGK_SALTY] Required for invasion of epithelial cells. Could be involved in protein secretion. |
| - | <div style="background-color:#fffaf0;">
| + | |
| - | == Publication Abstract from PubMed ==
| + | |
| - | The type III secretion (T3S) injectisome is a specialized protein nanomachine that is critical for the pathogenicity of many Gram-negative bacteria, including purveyors of plague, typhoid fever, whooping cough, sexually transmitted infections and major nosocomial infections. This syringe-shaped 3.5-MDa macromolecular assembly spans both bacterial membranes and that of the infected host cell. The internal channel formed by the injectisome allows for the direct delivery of partially unfolded virulence effectors into the host cytoplasm. The structural foundation of the injectisome is the basal body, a molecular lock-nut structure composed predominantly of three proteins that form highly oligomerized concentric rings spanning the inner and outer membranes. Here we present the structure of the prototypical Salmonella enterica serovar Typhimurium pathogenicity island 1 basal body, determined using single-particle cryo-electron microscopy, with the inner-membrane-ring and outer-membrane-ring oligomers defined at 4.3 A and 3.6 A resolution, respectively. This work presents the first, to our knowledge, high-resolution structural characterization of the major components of the basal body in the assembled state, including that of the widespread class of outer-membrane portals known as secretins.
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| - | Near-atomic-resolution cryo-EM analysis of the Salmonella T3S injectisome basal body.,Worrall LJ, Hong C, Vuckovic M, Deng W, Bergeron JR, Majewski DD, Huang RK, Spreter T, Finlay BB, Yu Z, Strynadka NC Nature. 2016 Dec 14. doi: 10.1038/nature20576. PMID:27974800<ref>PMID:27974800</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 5tcp" style="background-color:#fffaf0;"></div>
| + | |
| - | == References ==
| + | |
| - | <references/>
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| | __TOC__ | | __TOC__ |
| | </SX> | | </SX> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Salmonella enterica subsp. enterica serovar typhimurium]] | + | [[Category: Salmonella enterica subsp. enterica serovar Typhimurium]] |
| - | [[Category: Bergeron, J R.C]] | + | [[Category: Bergeron JRC]] |
| - | [[Category: Hong, C]] | + | [[Category: Hong C]] |
| - | [[Category: Huang, R K]] | + | [[Category: Huang RK]] |
| - | [[Category: Strynadka, N C.J]] | + | [[Category: Strynadka NCJ]] |
| - | [[Category: Vuckovic, M]] | + | [[Category: Vuckovic M]] |
| - | [[Category: Worrall, L J]] | + | [[Category: Worrall LJ]] |
| - | [[Category: Yu, Z]] | + | [[Category: Yu Z]] |
| - | [[Category: Bacterial]]
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| - | [[Category: Injectisome]]
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| - | [[Category: Membrane protein]]
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| - | [[Category: Secretion]]
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