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1f6m

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[[Image:1f6m.gif|left|200px]]
 
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{{Structure
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==CRYSTAL STRUCTURE OF A COMPLEX BETWEEN THIOREDOXIN REDUCTASE, THIOREDOXIN, AND THE NADP+ ANALOG, AADP+==
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|PDB= 1f6m |SIZE=350|CAPTION= <scene name='initialview01'>1f6m</scene>, resolution 2.95&Aring;
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<StructureSection load='1f6m' size='340' side='right'caption='[[1f6m]], [[Resolution|resolution]] 2.95&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene> and <scene name='pdbligand=3AA:3-AMINOPYRIDINE-ADENINE DINUCLEOTIDE PHOSPHATE'>3AA</scene>
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<table><tr><td colspan='2'>[[1f6m]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F6M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1F6M FirstGlance]. <br>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Thioredoxin-disulfide_reductase Thioredoxin-disulfide reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.8.1.9 1.8.1.9]
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.95&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3AA:3-AMINOPYRIDINE-ADENINE+DINUCLEOTIDE+PHOSPHATE'>3AA</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1f6m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f6m OCA], [https://pdbe.org/1f6m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1f6m RCSB], [https://www.ebi.ac.uk/pdbsum/1f6m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1f6m ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TRXB_ECOLI TRXB_ECOLI]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f6/1f6m_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1f6m ConSurf].
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<div style="clear:both"></div>
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'''CRYSTAL STRUCTURE OF A COMPLEX BETWEEN THIOREDOXIN REDUCTASE, THIOREDOXIN, AND THE NADP+ ANALOG, AADP+'''
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==See Also==
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*[[Thioredoxin 3D structures|Thioredoxin 3D structures]]
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*[[Thioredoxin reductase 3D structures|Thioredoxin reductase 3D structures]]
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==Overview==
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__TOC__
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In thioredoxin reductase (TrxR) from Escherichia coli, cycles of reduction and reoxidation of the flavin adenine dinucleotide (FAD) cofactor depend on rate-limiting rearrangements of the FAD and NADPH (reduced form of nicotinamide adenine dinucleotide phosphate) domains. We describe the structure of the flavin-reducing conformation of E. coli TrxR at a resolution of 3.0 angstroms. The orientation of the two domains permits reduction of FAD by NADPH and oxidation of the enzyme dithiol by the protein substrate, thioredoxin. The alternate conformation, described by Kuriyan and co-workers, permits internal transfer of reducing equivalents from reduced FAD to the active-site disulfide. Comparison of these structures demonstrates that switching between the two conformations involves a "ball-and-socket" motion in which the pyridine nucleotide-binding domain rotates by 67 degrees.
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</StructureSection>
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==About this Structure==
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1F6M is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F6M OCA].
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==Reference==
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Twists in catalysis: alternating conformations of Escherichia coli thioredoxin reductase., Lennon BW, Williams CH Jr, Ludwig ML, Science. 2000 Aug 18;289(5482):1190-4. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10947986 10947986]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Thioredoxin-disulfide reductase]]
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[[Category: Lennon BW]]
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[[Category: Jr., C H.Williams.]]
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[[Category: Ludwig ML]]
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[[Category: Lennon, B W.]]
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[[Category: Williams Jr CH]]
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[[Category: Ludwig, M L.]]
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[[Category: 3AA]]
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[[Category: FAD]]
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[[Category: alternate conformation]]
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[[Category: domain motion]]
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[[Category: electron transport]]
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[[Category: fad]]
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[[Category: nadp]]
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[[Category: redox-active center]]
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[[Category: ternary complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:05:08 2008''
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Current revision

CRYSTAL STRUCTURE OF A COMPLEX BETWEEN THIOREDOXIN REDUCTASE, THIOREDOXIN, AND THE NADP+ ANALOG, AADP+

PDB ID 1f6m

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