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1hqc

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[[Image:1hqc.gif|left|200px]]
 
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{{Structure
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==STRUCTURE OF RUVB FROM THERMUS THERMOPHILUS HB8==
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|PDB= 1hqc |SIZE=350|CAPTION= <scene name='initialview01'>1hqc</scene>, resolution 3.2&Aring;
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<StructureSection load='1hqc' size='340' side='right'caption='[[1hqc]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=ADE:ADENINE'>ADE</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>
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<table><tr><td colspan='2'>[[1hqc]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HQC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HQC FirstGlance]. <br>
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Adenosinetriphosphatase Adenosinetriphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.3 3.6.1.3] </span>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
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|GENE= RUVB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=274 Thermus thermophilus])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADE:ADENINE'>ADE</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hqc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hqc OCA], [https://pdbe.org/1hqc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hqc RCSB], [https://www.ebi.ac.uk/pdbsum/1hqc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hqc ProSAT]</span></td></tr>
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|RELATEDENTRY=
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hqc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hqc OCA], [http://www.ebi.ac.uk/pdbsum/1hqc PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1hqc RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/RUVB_THET8 RUVB_THET8] The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing. RuvB is a Mg(2+)-dependent, DNA-dependent ATPase with an equal preference for supercoiled and linear duplex DNA. It can promote Holliday junction migration alone.<ref>PMID:10485292</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hq/1hqc_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1hqc ConSurf].
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<div style="clear:both"></div>
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'''STRUCTURE OF RUVB FROM THERMUS THERMOPHILUS HB8'''
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==See Also==
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*[[Helicase 3D structures|Helicase 3D structures]]
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== References ==
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==Overview==
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<references/>
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We report here the crystal structure of the RuvB motor protein from Thermus thermophilus HB8, which drives branch migration of the Holliday junction during homologous recombination. RuvB has a crescent-like architecture consisting of three consecutive domains, the first two of which are involved in ATP binding and hydrolysis. DNA is likely to interact with a large basic cleft, which encompasses the ATP-binding pocket and domain boundaries, whereas the junction-recognition protein RuvA may bind a flexible beta-hairpin protruding from the N-terminal domain. The structures of two subunits, related by a noncrystallographic pseudo-2-fold axis, imply that conformational changes of motor protein coupled with ATP hydrolysis may reflect motility essential for its translocation around double-stranded DNA.
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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1HQC is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HQC OCA].
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==Reference==
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Crystal structure of the Holliday junction migration motor protein RuvB from Thermus thermophilus HB8., Yamada K, Kunishima N, Mayanagi K, Ohnishi T, Nishino T, Iwasaki H, Shinagawa H, Morikawa K, Proc Natl Acad Sci U S A. 2001 Feb 13;98(4):1442-7. Epub 2001 Feb 6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11171970 11171970]
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[[Category: Adenosinetriphosphatase]]
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[[Category: Single protein]]
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[[Category: Thermus thermophilus]]
[[Category: Thermus thermophilus]]
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[[Category: Iwasaki, H.]]
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[[Category: Iwasaki H]]
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[[Category: Kunishima, N.]]
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[[Category: Kunishima N]]
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[[Category: Mayanagi, K.]]
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[[Category: Mayanagi K]]
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[[Category: Morikawa, K.]]
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[[Category: Morikawa K]]
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[[Category: Yamada, K.]]
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[[Category: Yamada K]]
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[[Category: complex with nucleotide]]
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[[Category: extended aaa-atpase domain]]
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[[Category: ruvb]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:09:00 2008''
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Current revision

STRUCTURE OF RUVB FROM THERMUS THERMOPHILUS HB8

PDB ID 1hqc

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