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1imj

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(New page: 200px<br /> <applet load="1imj" size="450" color="white" frame="true" align="right" spinBox="true" caption="1imj, resolution 2.20&Aring;" /> '''CRYSTAL STRUCTURE O...)
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[[Image:1imj.gif|left|200px]]<br />
 
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<applet load="1imj" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1imj, resolution 2.20&Aring;" />
 
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'''CRYSTAL STRUCTURE OF THE HUMAN CCG1/TAFII250-INTERACTING FACTOR B (CIB)'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF THE HUMAN CCG1/TAFII250-INTERACTING FACTOR B (CIB)==
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The general transcription initiation factor TFIID and its interactors play, critical roles in regulating the transcription from both naked and, chromatin DNA. We have isolated a novel TFIID interactor that we denoted, as CCG1/TAF(II)250-interacting factor B (CIB). We show here that CIB, activates transcription. To further understand the function of this, protein, we determined its crystal structure at 2.2-Angstroms resolution., The tertiary structure of CIB reveals an alpha/beta-hydrolase fold that, resembles structures in the prokaryotic alpha/beta-hydrolase family, proteins. It is not similar in structure or primary sequence to any, eukaryotic transcription or chromatin factors that have been reported to, date. CIB possesses a conserved catalytic triad that is found in other, alpha/beta-hydrolases, and our in vitro studies confirmed that it bears, hydrolase activity. However, CIB differs from other alpha/beta-hydrolases, in that it lacks a binding site excursion, which facilitates the substrate, selectivity of the other alpha/beta-hydrolases. Further functional, characterization of CIB based on its tertiary structure and through, biochemical studies may provide novel insights into the mechanisms that, regulate eukaryotic transcription.
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<StructureSection load='1imj' size='340' side='right'caption='[[1imj]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1imj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IMJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IMJ FirstGlance]. <br>
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1IMJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1IMJ OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1imj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1imj OCA], [https://pdbe.org/1imj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1imj RCSB], [https://www.ebi.ac.uk/pdbsum/1imj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1imj ProSAT]</span></td></tr>
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The crystal structure of CCG1/TAF(II)250-interacting factor B (CIB)., Padmanabhan B, Kuzuhara T, Adachi N, Horikoshi M, J Biol Chem. 2004 Mar 5;279(10):9615-24. Epub 2003 Dec 11. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14672934 14672934]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ABHEB_HUMAN ABHEB_HUMAN] Has hydrolase activity towards p-nitrophenyl butyrate (in vitro). May activate transcription.<ref>PMID:14672934</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/im/1imj_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1imj ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Horikoshi, M.]]
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[[Category: Horikoshi M]]
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[[Category: Kuzuhara, T.]]
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[[Category: Kuzuhara T]]
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[[Category: Padmanabhan, B.]]
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[[Category: Padmanabhan B]]
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[[Category: SO4]]
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[[Category: alpha/beta hydrolase]]
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[[Category: ccg1 interactor]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 17:31:54 2007''
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Current revision

CRYSTAL STRUCTURE OF THE HUMAN CCG1/TAFII250-INTERACTING FACTOR B (CIB)

PDB ID 1imj

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