1jv1

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[[Image:1jv1.jpg|left|200px]]<br /><applet load="1jv1" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1jv1, resolution 1.90&Aring;" />
 
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'''CRYSTAL STRUCTURE OF HUMAN AGX1 COMPLEXED WITH UDPGLCNAC'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF HUMAN AGX1 COMPLEXED WITH UDPGLCNAC==
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The recently published human genome with its relatively modest number of, genes has highlighted the importance of post-transcriptional and, post-translational modifications, such as alternative splicing or, glycosylation, in generating the complexities of human biology. The human, UDP-N-acetylglucosamine (UDPGlcNAc) pyrophosphorylases AGX1 and AGX2, which differ in sequence by an alternatively spliced 17 residue peptide, are key enzymes synthesizing UDPGlcNAc, an essential precursor for protein, glycosylation. To better understand the catalytic mechanism of these, enzymes and the role of the alternatively spliced segment, we have solved, the crystal structures of AGX1 and AGX2 in complexes with UDPGlcNAc (at, 1.9 and 2.4 A resolution, respectively) and UDPGalNAc (at 2.2 and 2.3 A, resolution, respectively). Comparison with known structures classifies, AGX1 and AGX2 as two new members of the SpsA-GnT I Core superfamily and, together with mutagenesis analysis, helps identify residues critical for, catalysis. Most importantly, our combined structural and biochemical data, provide evidence for a change in the oligomeric assembly accompanied by a, significant modification of the active site architecture, a result, suggesting that the two isoforms generated by alternative splicing may, have distinct catalytic properties.
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<StructureSection load='1jv1' size='340' side='right'caption='[[1jv1]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1jv1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JV1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JV1 FirstGlance]. <br>
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1JV1 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=UD1:'>UD1</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JV1 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=UD1:URIDINE-DIPHOSPHATE-N-ACETYLGLUCOSAMINE'>UD1</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jv1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jv1 OCA], [https://pdbe.org/1jv1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jv1 RCSB], [https://www.ebi.ac.uk/pdbsum/1jv1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jv1 ProSAT]</span></td></tr>
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Crystal structures of two human pyrophosphorylase isoforms in complexes with UDPGlc(Gal)NAc: role of the alternatively spliced insert in the enzyme oligomeric assembly and active site architecture., Peneff C, Ferrari P, Charrier V, Taburet Y, Monnier C, Zamboni V, Winter J, Harnois M, Fassy F, Bourne Y, EMBO J. 2001 Nov 15;20(22):6191-202. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11707391 11707391]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/UAP1_HUMAN UAP1_HUMAN] Converts UDP and GlcNAc-1-P into UDP-GlcNAc, and UDP and GalNAc-1-P into UDP-GalNAc. Isoform AGX1 has 2 to 3 times higher activity towards GalNAc-1-P, while isoform AGX2 has 8 times more activity towards GlcNAc-1-P.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jv/1jv1_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jv1 ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Bourne, Y.]]
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[[Category: Bourne Y]]
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[[Category: Peneff, C.]]
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[[Category: Peneff C]]
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[[Category: UD1]]
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[[Category: alternative splicing]]
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[[Category: nucleotidyltransferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:09:37 2008''
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Current revision

CRYSTAL STRUCTURE OF HUMAN AGX1 COMPLEXED WITH UDPGLCNAC

PDB ID 1jv1

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