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1k9u

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(New page: 200px<br /><applet load="1k9u" size="450" color="white" frame="true" align="right" spinBox="true" caption="1k9u, resolution 1.75&Aring;" /> '''Crystal Structure of...)
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[[Image:1k9u.jpg|left|200px]]<br /><applet load="1k9u" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1k9u, resolution 1.75&Aring;" />
 
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'''Crystal Structure of the Calcium-Binding Pollen Allergen Phl p 7 (Polcalcin) at 1.75 Angstroem'''<br />
 
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==Overview==
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==Crystal Structure of the Calcium-Binding Pollen Allergen Phl p 7 (Polcalcin) at 1.75 Angstroem==
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The timothy grass pollen allergen Phl p 7 assembles most of the IgE, epitopes of a novel family of 2 EF-hand calcium-binding proteins and, therefore represents a diagnostic marker allergen and vaccine candidate, for immunotherapy. Here we report the first three-dimensional structure of, a representative of the 2 EF-hand allergen family, Phl p 7, in the, calcium-bound form. The protein occurs as a novel dimer assembly with, unique features: in contrast to well known EF-hand proteins such as, calmodulin, parvalbumin or the S100 proteins, Phl p 7 adopts an extended, conformation. Two protein monomers assemble in a head-to-tail arrangement, with domain-swapped EF-hand pairing. The intertwined dimer adopts a, barrel-like structure with an extended hydrophobic cavity providing a, ligand-binding site. Calcium binding acts as a conformational switch, between an open and a closed dimeric form of Phl p 7. These findings are, interesting in the context of lipid- and calcium-dependent pollen tube, growth. Furthermore, the structure of Phl p 7 allows for the rational, development of vaccine strategies for treatment of sensitized allergic, patients.
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<StructureSection load='1k9u' size='340' side='right'caption='[[1k9u]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1k9u]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Phleum_pratense Phleum pratense]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K9U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1K9U FirstGlance]. <br>
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1K9U is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Phleum_pratense Phleum pratense] with SO4 and CA as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1K9U OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1k9u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k9u OCA], [https://pdbe.org/1k9u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1k9u RCSB], [https://www.ebi.ac.uk/pdbsum/1k9u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1k9u ProSAT]</span></td></tr>
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The cross-reactive calcium-binding pollen allergen, Phl p 7, reveals a novel dimer assembly., Verdino P, Westritschnig K, Valenta R, Keller W, EMBO J. 2002 Oct 1;21(19):5007-16. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12356717 12356717]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/POLC7_PHLPR POLC7_PHLPR]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k9/1k9u_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1k9u ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Phleum pratense]]
[[Category: Phleum pratense]]
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[[Category: Single protein]]
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[[Category: Keller W]]
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[[Category: Keller, W.]]
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[[Category: Valenta R]]
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[[Category: Valenta, R.]]
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[[Category: Verdino P]]
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[[Category: Verdino, P.]]
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[[Category: Westritschnig K]]
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[[Category: Westritschnig, K.]]
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[[Category: CA]]
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[[Category: SO4]]
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[[Category: calcium-binding]]
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[[Category: cross-reactivity]]
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[[Category: ef-hand]]
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[[Category: pollen allergen]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 18:59:08 2007''
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Current revision

Crystal Structure of the Calcium-Binding Pollen Allergen Phl p 7 (Polcalcin) at 1.75 Angstroem

PDB ID 1k9u

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