1kgn

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[[Image:1kgn.jpg|left|200px]]<br /><applet load="1kgn" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1kgn, resolution 1.85&Aring;" />
 
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'''R2F from Corynebacterium Ammoniagenes in its oxidised, Fe containing, form'''<br />
 
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==Overview==
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==R2F from Corynebacterium Ammoniagenes in its oxidised, Fe containing, form==
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Ribonucleotide reductase (RNR) is the enzyme performing de novo production of the four deoxyribonucleotides needed for DNA synthesis. All mammals as well as some prokaryotes express the class I enzyme which is an alpha(2)beta(2) protein. The smaller of the homodimers, denoted R2, contains a di-iron carboxylate site which, upon reaction with molecular oxygen, generates a stable tyrosyl radical needed for catalysis. The three-dimensional structure of the oxidized class Ib RNR R2 from Corynebacterium ammoniagenes has been determined at 1.85 A resolution and refined to an R-value of 15.8% (R(free) = 21.3%). In addition, structures of both the reduced iron-containing, and manganese-substituted protein have been solved. The C. ammoniagenes R2 has been proposed to be manganese-dependent. The present structure provides evidence that manganese is not oxidized by the protein, in agreement with recent biochemical data, and that no obvious structural abnormalities are seen in the oxidized and reduced iron-containing forms, giving further support that the protein is indeed an iron-dependent RNR R2. The di-manganese structure also provides an explanation for the magnetic properties of this site. The structure of the oxidized C. ammoniagenes R2 also reveals an additional water molecule bridging the radical and the iron site, which has not previously been seen in any other R2 structure and which might have important mechanistic implications.
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<StructureSection load='1kgn' size='340' side='right'caption='[[1kgn]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1kgn]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Corynebacterium_ammoniagenes Corynebacterium ammoniagenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KGN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KGN FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kgn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kgn OCA], [https://pdbe.org/1kgn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kgn RCSB], [https://www.ebi.ac.uk/pdbsum/1kgn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kgn ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/O69274_CORAM O69274_CORAM] Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides (By similarity).[PIRNR:PIRNR000355]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kg/1kgn_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1kgn ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1KGN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Corynebacterium_ammoniagenes Corynebacterium ammoniagenes] with <scene name='pdbligand=FE:'>FE</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KGN OCA].
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*[[Ribonucleotide reductase 3D structures|Ribonucleotide reductase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Crystal structure of the di-iron/radical protein of ribonucleotide reductase from Corynebacterium ammoniagenes., Hogbom M, Huque Y, Sjoberg BM, Nordlund P, Biochemistry. 2002 Jan 29;41(4):1381-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11802741 11802741]
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[[Category: Corynebacterium ammoniagenes]]
[[Category: Corynebacterium ammoniagenes]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Hogbom, M.]]
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[[Category: Hogbom M]]
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[[Category: Huque, Y.]]
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[[Category: Huque Y]]
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[[Category: Nordlund, P.]]
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[[Category: Nordlund P]]
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[[Category: Sjoberg, B M.]]
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[[Category: Sjoberg BM]]
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[[Category: FE]]
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[[Category: arm exchange]]
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[[Category: helix bundle]]
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[[Category: radical protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:33:58 2008''
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Current revision

R2F from Corynebacterium Ammoniagenes in its oxidised, Fe containing, form

PDB ID 1kgn

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