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1kwh

From Proteopedia

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[[Image:1kwh.jpg|left|200px]]
 
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{{Structure
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==Structure Analysis AlgQ2, a Macromolecule(alginate)-Binding Periplasmic Protein of Sphingomonas sp. A1.==
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|PDB= 1kwh |SIZE=350|CAPTION= <scene name='initialview01'>1kwh</scene>, resolution 2.00&Aring;
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<StructureSection load='1kwh' size='340' side='right'caption='[[1kwh]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=CA:CALCIUM ION'>CA</scene>
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<table><tr><td colspan='2'>[[1kwh]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Sphingomonas_sp._A1 Sphingomonas sp. A1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KWH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KWH FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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|GENE= algQ2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=28214 Sphingomonas sp.])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kwh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kwh OCA], [https://pdbe.org/1kwh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kwh RCSB], [https://www.ebi.ac.uk/pdbsum/1kwh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kwh ProSAT]</span></td></tr>
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</table>
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'''Structure Analysis AlgQ2, a Macromolecule(alginate)-Binding Periplasmic Protein of Sphingomonas sp. A1.'''
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== Function ==
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[https://www.uniprot.org/uniprot/Q9KWT5_SPHSX Q9KWT5_SPHSX]
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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Sphingomonas sp. A1 possesses a high molecular mass (average 25,700 Da) alginate uptake system mediated by a novel pit-dependent ABC transporter. The X-ray crystallographic structure of AlgQ2 (57,200 Da), an alginate-binding protein in the system, was determined by the multiple isomorphous replacement method and refined at 2.0 A resolution with a final R-factor of 18.3% for 15 to 2.0 A resolution data. The refined structure of AlgQ2 was comprised of 492 amino acid residues, 172 water molecules, and one calcium ion. AlgQ2 was composed of two globular domains with a deep cleft between them, which is expected to be the alginate-binding site. The overall structure is basically similar to that of maltose/maltodextrin-binding protein, except for the presence of an N2-subdomain. The entire calcium ion-binding site is similar to the site in the EF-hand motif, but comprises a ten residue loop. This calcium ion-binding site is about 40 A away from the alginate-binding site.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kw/1kwh_consurf.spt"</scriptWhenChecked>
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1KWH is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Sphingomonas_sp. Sphingomonas sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KWH OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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Crystal structure of AlgQ2, a macromolecule (alginate)-binding protein of Sphingomonas sp. A1 at 2.0A resolution., Momma K, Mikami B, Mishima Y, Hashimoto W, Murata K, J Mol Biol. 2002 Mar 8;316(5):1051-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11884143 11884143]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1kwh ConSurf].
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[[Category: Single protein]]
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<div style="clear:both"></div>
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[[Category: Sphingomonas sp.]]
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__TOC__
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[[Category: Hashimoto, W.]]
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</StructureSection>
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[[Category: Mikami, B.]]
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[[Category: Large Structures]]
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[[Category: Mishima, Y.]]
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[[Category: Sphingomonas sp. A1]]
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[[Category: Momma, K.]]
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[[Category: Hashimoto W]]
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[[Category: Murata, K.]]
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[[Category: Mikami B]]
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[[Category: CA]]
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[[Category: Mishima Y]]
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[[Category: alginate]]
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[[Category: Momma K]]
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[[Category: binding protein]]
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[[Category: Murata K]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:23:07 2008''
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Current revision

Structure Analysis AlgQ2, a Macromolecule(alginate)-Binding Periplasmic Protein of Sphingomonas sp. A1.

PDB ID 1kwh

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