1mez

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(New page: 200px<br /><applet load="1mez" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mez, resolution 2.4&Aring;" /> '''Structure of the Reco...)
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[[Image:1mez.jpg|left|200px]]<br /><applet load="1mez" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1mez, resolution 2.4&Aring;" />
 
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'''Structure of the Recombinant Mouse-Muscle Adenylosuccinate Synthetase Complexed with SAMP, GDP, SO4(2-), and Mg(2+)'''<br />
 
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==Overview==
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==Structure of the Recombinant Mouse-Muscle Adenylosuccinate Synthetase Complexed with SAMP, GDP, SO4(2-), and Mg(2+)==
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Adenylosuccinate synthetase governs the committed step of AMP, biosynthesis, the generation of 6-phosphoryl-IMP from GTP and IMP followed, by the formation of adenylosuccinate from 6-phosphoryl-IMP and, l-aspartate. The enzyme is subject to feedback inhibition by AMP and, adenylosuccinate, but crystallographic complexes of the mouse muscle, synthetase presented here infer mechanisms of inhibition that involve, potentially synergistic ligand combinations. AMP alone adopts the, productive binding mode of IMP and yet stabilizes the active site in a, conformation that favors the binding of Mg(2+)-IMP to the GTP pocket. On, the other hand, AMP, in the presence of GDP, orthophosphate, and Mg(2+), adopts the binding mode of adenylosuccinate. Depending on circumstances, then, AMP behaves as an analogue of IMP or as an analogue of, adenylosuccinate. The complex of adenylosuccinate.GDP.Mg(2+).sulfate, the, first structure of an adenylosuccinate-bound synthetase, reveals, significant geometric distortions and tight nonbonded contacts relevant to, the proposed catalytic mechanism. Adenylosuccinate forms from, 6-phosphoryl-IMP and l-aspartate by the movement of the purine ring into, the alpha-amino group of l-aspartate.
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<StructureSection load='1mez' size='340' side='right'caption='[[1mez]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1mez]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MEZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MEZ FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2SA:2-[9-(3,4-DIHYDROXY-5-PHOSPHONOOXYMETHYL-TETRAHYDRO-FURAN-2-YL)-9H-PURIN-6-YLAMINO]-SUCCINIC+ACID'>2SA</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mez FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mez OCA], [https://pdbe.org/1mez PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mez RCSB], [https://www.ebi.ac.uk/pdbsum/1mez PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mez ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PURA1_MOUSE PURA1_MOUSE] Component of the purine nucleotide cycle (PNC), which interconverts IMP and AMP to regulate the nucleotide levels in various tissues, and which contributes to glycolysis and ammoniagenesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP.<ref>PMID:12482871</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/me/1mez_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mez ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1MEZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with MG, SO4, GDP and 2SA as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Adenylosuccinate_synthase Adenylosuccinate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.4.4 6.3.4.4] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1MEZ OCA].
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*[[Adenylosuccinate synthetase 3D structures|Adenylosuccinate synthetase 3D structures]]
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== References ==
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==Reference==
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<references/>
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Feedback inhibition and product complexes of recombinant mouse muscle adenylosuccinate synthetase., Iancu CV, Borza T, Fromm HJ, Honzatko RB, J Biol Chem. 2002 Oct 25;277(43):40536-43. Epub 2002 Aug 16. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12186864 12186864]
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__TOC__
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[[Category: Adenylosuccinate synthase]]
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Single protein]]
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[[Category: Borza T]]
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[[Category: Borza, T.]]
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[[Category: Fromm HJ]]
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[[Category: Fromm, H.J.]]
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[[Category: Honzatko RB]]
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[[Category: Honzatko, R.B.]]
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[[Category: Iancu CV]]
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[[Category: Iancu, C.V.]]
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[[Category: 2SA]]
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[[Category: GDP]]
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[[Category: MG]]
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[[Category: SO4]]
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[[Category: gtp-binding]]
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[[Category: multigene family]]
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[[Category: purine biosynthesis]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 21:22:39 2007''
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Current revision

Structure of the Recombinant Mouse-Muscle Adenylosuccinate Synthetase Complexed with SAMP, GDP, SO4(2-), and Mg(2+)

PDB ID 1mez

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