1mw2

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[[Image:1mw2.jpg|left|200px]]<br /><applet load="1mw2" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1mw2, resolution 2.10&Aring;" />
 
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'''Amylosucrase soaked with 100mM sucrose'''<br />
 
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==Overview==
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==Amylosucrase soaked with 100mM sucrose==
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The glucosyltransferase amylosucrase is structurally quite similar to the hydrolase alpha-amylase. How this switch in functionality is achieved is an important and fundamental question. The inactive E328Q amylosucrase variant has been co-crystallized with maltoheptaose, and the structure was determined by x-ray crystallography to 2.2 A resolution, revealing a maltoheptaose binding site in the B'-domain somewhat distant from the active site. Additional soaking of these crystals with maltoheptaose resulted in replacement of Tris in the active site with maltoheptaose, allowing the mapping of the -1 to +5 binding subsites. Crystals of amylosucrase were soaked with sucrose at different concentrations. The structures at approximately 2.1 A resolution revealed three new binding sites of different affinity. The highest affinity binding site is close to the active site but is not in the previously identified substrate access channel. Allosteric regulation seems necessary to facilitate access from this binding site. The structures show the pivotal role of the B'-domain in the transferase reaction. Based on these observations, an extension of the hydrolase reaction mechanism valid for this enzyme can be proposed. In this mechanism, the glycogen-like polymer is bound in the widest access channel to the active site. The polymer binding introduces structural changes that allow sucrose to migrate from its binding site into the active site and displace the polymer.
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<StructureSection load='1mw2' size='340' side='right'caption='[[1mw2]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1mw2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Neisseria_polysaccharea Neisseria polysaccharea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MW2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MW2 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=PRD_900003:sucrose'>PRD_900003</scene>, <scene name='pdbligand=PRD_900010:alpha-maltotetraose'>PRD_900010</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mw2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mw2 OCA], [https://pdbe.org/1mw2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mw2 RCSB], [https://www.ebi.ac.uk/pdbsum/1mw2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mw2 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/AMYS_NEIPO AMYS_NEIPO] Catalyzes the synthesis of alpha-glucan from sucrose. Catalyzes, in addition, sucrose hydrolysis, maltose and maltotriose synthesis by successive transfers of the glucosyl moiety of sucrose onto the released glucose, and finally turanose and trehalulose synthesis, these two sucrose isomers being obtained by glucosyl transfer onto fructose.<ref>PMID:9882648</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mw/1mw2_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mw2 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1MW2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Neisseria_polysaccharea Neisseria polysaccharea] with <scene name='pdbligand=SUC:'>SUC</scene> and <scene name='pdbligand=TRS:'>TRS</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Amylosucrase Amylosucrase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.4 2.4.1.4] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MW2 OCA].
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*[[Amylase 3D structures|Amylase 3D structures]]
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== References ==
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==Reference==
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<references/>
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Oligosaccharide and sucrose complexes of amylosucrase. Structural implications for the polymerase activity., Skov LK, Mirza O, Sprogoe D, Dar I, Remaud-Simeon M, Albenne C, Monsan P, Gajhede M, J Biol Chem. 2002 Dec 6;277(49):47741-7. Epub 2002 Oct 2. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12364331 12364331]
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__TOC__
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[[Category: Amylosucrase]]
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Neisseria polysaccharea]]
[[Category: Neisseria polysaccharea]]
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[[Category: Single protein]]
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[[Category: Albenne C]]
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[[Category: Albenne, C.]]
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[[Category: Dar I]]
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[[Category: Dar, I.]]
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[[Category: Gajhede M]]
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[[Category: Gajhede, M.]]
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[[Category: Mirza O]]
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[[Category: Mirza, O.]]
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[[Category: Monsan P]]
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[[Category: Monsan, P.]]
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[[Category: Remaud-Simeon M]]
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[[Category: Remaud-Simeon, M.]]
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[[Category: Skov LK]]
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[[Category: Skov, L K.]]
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[[Category: Sprogoe D]]
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[[Category: Sprogoe, D.]]
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[[Category: SUC]]
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[[Category: TRS]]
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[[Category: (beta-alpha)8 barrel]]
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[[Category: protein-sugar complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:59:45 2008''
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Current revision

Amylosucrase soaked with 100mM sucrose

PDB ID 1mw2

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